Sandhu S S, Kinghorn J R, Rajak R C, Unkles S E
Department of Biological Science, Rani Durgawati University, Jabalpur, India.
Indian J Exp Biol. 2001 Jul;39(7):650-3.
An heterologous transformation system for entomopathogenic fungi B. bassiana and M. anisopliae was developed based on the use of A. nidulans nitrate reductase gene (niaD). B. bassiana and M. anisopliae niaD stable mutants were selected by treatment of protoplast with ethane methane sulphonate (EMS) and regenerated on chlorate medium. The cloned gene was capable of transforming B. bassiana and M. anisopliae at a frequency of 5.8 to 20 transformants per microg of DNA. Most of them were mitotically stable.
基于构巢曲霉硝酸还原酶基因（niaD），开发了一种用于昆虫病原真菌球孢白僵菌和金龟子绿僵菌的异源转化系统。通过用乙烷甲磺酸盐（EMS）处理原生质体并在氯酸盐培养基上再生，筛选出球孢白僵菌和金龟子绿僵菌的niaD稳定突变体。克隆的基因能够以每微克DNA产生5.8至20个转化体的频率转化球孢白僵菌和金龟子绿僵菌。它们中的大多数在有丝分裂时是稳定的。
