基于构巢曲霉硝酸还原酶基因构建轮枝镰孢（Lecanicillium spp.）（半知菌亚门：丝孢纲）转化系统。
Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans.
机构信息
Fungal Biotechnology and Invertebrate Pathology Laboratory, Department of Biological Sciences, R.D. University, Jabalpur, India.
出版信息
Indian J Microbiol. 2011 Jul;51(3):390-5. doi: 10.1007/s12088-011-0106-5. Epub 2011 Jan 25.
Abstract
A heterologous transformation system was developed for V. lecanii based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. Mutant no. 01 and 04 was chosen for the transformation experiments. Plasmid pBT was used as transformation vector containing the Aspergillus nidulans nitrate reductase gene. A frequency of approximately 3 transformants/μg DNA was obtained using the circular vector pBT. The establishment of a transformation system for V. lecanii is fundamental for genetic manipulation of this microorganism.
摘要
建立了基于硝酸还原酶突变体互补的卷枝毛霉异源转化系统。通过比较其他导致对氯酸盐抗性的突变体，筛选出对氯酸盐具有抗性的突变体，抗性突变体的获得率为 23.24%。选择突变体 01 和 04 进行转化实验。质粒 pBT 被用作转化载体，其中含有 Aspergillus nidulans 硝酸还原酶基因。使用环形载体 pBT 获得了约 3 个转化体/μg DNA 的频率。卷枝毛霉转化系统的建立是对该微生物进行遗传操作的基础。
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