利用编码硝酸还原酶的黑曲霉niaD基因对丝状真菌藤仓赤霉进行转化。

Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.

作者信息

Sànchez-Fernàndez R, Unkles S E, Campbell E I, Macro J A, Cerdà-Olmedo E, Kinghorn J R

机构信息

Departamento de Genetica y Biotecnia, Universidad de Sevilla, Spain.

出版信息

Mol Gen Genet. 1991 Feb;225(2):231-3. doi: 10.1007/BF00269853.

DOI:10.1007/BF00269853

PMID:2005864

Abstract

A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.

摘要

构建了一种基于黑曲霉硝酸还原酶基因（niaD）的藤仓赤霉菌转化系统。通过筛选在以硝酸盐作为唯一氮源时不生长的诱变细胞，获得了一株携带非回复性niaD突变（niaD11）的菌株（命名为SG140）。当菌株SG140被转化为可利用硝酸盐时，观察到每微克DNA有1 - 2个转化体的转化频率。对niaD +转化体的Southern印迹分析表明，载体DNA序列已整合到染色体DNA中。结果证明黑曲霉niaD基因在藤仓赤霉菌中表达。