Coursol Sylvie, Pierre Jean-Noël, Vidal Jean, Grisvard Jeanine
Institut de Biotechnologie des Plantes, UMR 8618, Université Paris XI, Bâtiment 630, 91405 Orsay Cedex, France.
J Exp Bot. 2002 Jun;53(373):1521-4.
As a PLC activity was implicated in the light transduction pathway that controls C(4) photosynthesis in Digitaria sanguinalis, a full length PLC cDNA (DsPLC2) was cloned. The proteins encoded by the two possible open reading frames were produced in Escherichia coli; they both harbour a PLC activity but with different response to Ca(2+) concentration, and with different sensitivity to the PLC inhibitor U-73122.
由于磷脂酶C（PLC）活性与控制马唐属植物C4光合作用的光转导途径有关，因此克隆了全长PLC cDNA（DsPLC2）。由两个可能的开放阅读框编码的蛋白质在大肠杆菌中产生；它们都具有PLC活性，但对Ca(2+)浓度的反应不同，对PLC抑制剂U-73122的敏感性也不同。
