Zhai Shumei, Sui Zhenhua, Yang Aifang, Zhang Juren
School of Life Science, Shandong University, Jinan, 250100, P.R. China.
Biotechnol Lett. 2005 Jun;27(11):799-804. doi: 10.1007/s10529-005-5802-y.
A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) has been isolated from Zea mays by screening a cDNA library. The cDNA, designated ZmPLC, encodes a polypeptide of 586 amino acids, containing the catalytic X, Y and C2 domains found in all PI-PLCs from plants. Northern blot analysis showed that the expression of the ZmPLC gene in roots is up-regulated under conditions of high salt, dehydration, cold or low osmotic stress conditions. Recombinant ZmPLC protein was expressed in Esch- erichia coli, purified and used to produce polyclonal antibody, this polyclonal antibody is important for further studies to assess the ultimate function of the ZmPLC gene in plants.
通过筛选玉米的cDNA文库，分离出了一个编码磷酸肌醇特异性磷脂酶C（PI-PLC）的cDNA。该cDNA被命名为ZmPLC，编码一个由586个氨基酸组成的多肽，含有在所有植物PI-PLC中都存在的催化X、Y和C2结构域。Northern杂交分析表明，ZmPLC基因在根中的表达在高盐、脱水、低温或低渗透胁迫条件下会上调。重组ZmPLC蛋白在大肠杆菌中表达、纯化并用于制备多克隆抗体，该多克隆抗体对于进一步研究评估ZmPLC基因在植物中的最终功能很重要。
