Oliveira Monteiro Lummy Maria, Vici Ana Claudia, Messias Josana Maria, Heinen Paulo Ricardo, Pinheiro Vanessa Elisa, Vargas Rechia Carem Gledes, Buckeridge Marcos S, Teixeira de Moraes Polizeli Maria de Lourdes
Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14049-900, Ribeirão Preto, SP, Brazil.
Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Bandeirantes Av., 3.900, 14040-900, Ribeirão Preto, SP, Brazil.
Biotechnol Rep (Amst). 2021 Apr 18;30:e00618. doi: 10.1016/j.btre.2021.e00618. eCollection 2021 Jun.
β-Glucosidases are a limiting factor in the conversion of cellulose to glucose for the subsequent ethanol production. Here, β-glucosidase production by was optimized using Composite Central Designs and Response Surface Methodologies from a medium designed. The coefficient of determination ( ) was 0.9960, -value was very high, and the lack of fit was found to be non-significant. This indicates a statistic valid and predictive result. enzymatic extract was successfully tested as an enzymatic cocktail in a mixture design using sugarcane bagasse, soybean hull and barley bagasse. We proved that the optimization of the β-glucosidase production and the application in hydrolysis using unexpansive biomass and agricultural wastes can be accomplished by means of statistical methodologies. The strategy presented here can be useful for the improvement of enzyme production and the hydrolysis process, arising as an alternative for bioeconomy.
β-葡萄糖苷酶是纤维素转化为葡萄糖以用于后续乙醇生产过程中的一个限制因素。在此，利用复合中心设计和响应面方法，从一种设计的培养基中对[具体微生物名称未给出]产生β-葡萄糖苷酶的条件进行了优化。决定系数（ ）为0.9960，F值非常高，且失拟不显著。这表明结果具有统计学有效性和预测性。在使用甘蔗渣、大豆皮和大麦渣的混合设计中，[具体微生物名称未给出]的酶提取物作为一种酶混合物成功进行了测试。我们证明，通过统计方法可以实现β-葡萄糖苷酶生产的优化以及在使用低成本生物质和农业废弃物进行水解中的应用。这里提出的策略对于提高酶产量和水解过程可能是有用的，可作为生物经济的一种替代方案。
