Ravindran Rajeev, Williams Gwilym A, Jaiswal Amit K
School of Food Science and Environmental Health, College of Sciences and Health, Dublin Institute of Technology, Cathal Brugha Street, D01 HV58 Dublin, Ireland.
School of Biological Sciences, College of Sciences and Health, Dublin Institute of Technology, Kevin Street, D08 NF82 Dublin, Ireland.
Foods. 2019 Nov 17;8(11):585. doi: 10.3390/foods8110585.
In this study, spent coffee waste (SCW) was used as the sole carbon source for xylanase production in solid state fermentation mode using . A Box-Behnken design was constructed using three parameters viz. temperature, initial moisture content, and log number of spores to determine the optimal fermentation condition. The best fermentation conditions for xylanase production were found to be incubation at 30 °C with an initial moisture content of 70% and using an inoculum of 6.5 × 10 spores/g of dry SCW. Furthermore, the design of experiments revealed that maintaining a medium composition of 0.2 g of yeast extract, 0.04 g of KHPO, and 0.03 g of MgSO increased xylanase production. Under optimised solid-state fermentation conditions an enzyme activity of 6495.6 IU/g of dry SCW was recorded, which was approximately 1.39-fold higher than that of control (4649 IU/g of dry SCW). The efficacy of the purified xylanase as a juice enrichment agent for strawberry, blueberry, and raspberry pulp was tested.
在本研究中，咖啡渣（SCW）被用作固态发酵模式下生产木聚糖酶的唯一碳源。使用温度、初始水分含量和孢子对数这三个参数构建了Box-Behnken设计，以确定最佳发酵条件。发现生产木聚糖酶的最佳发酵条件是在30℃下培养，初始水分含量为70%，接种量为6.5×10孢子/克干SCW。此外，实验设计表明，保持0.2克酵母提取物、0.04克KHPO和0.03克MgSO的培养基组成可提高木聚糖酶产量。在优化的固态发酵条件下，记录到的酶活性为6495.6 IU/克干SCW，比对照（4649 IU/克干SCW）高出约1.39倍。测试了纯化的木聚糖酶作为草莓、蓝莓和树莓果肉果汁浓缩剂的功效。
