五只葡萄牙猫免疫缺陷病毒毒株的系统发育分析。
Phylogenetic analysis of five Portuguese strains of FIV.
作者信息
Duarte A, Marques M I, Tavares L, Fevereiro M
机构信息
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA) - Faculdade de Medicina Veterinária da Universidade Técnica de Lisboa, Portugal.
出版信息
Arch Virol. 2002 May;147(5):1061-70. doi: 10.1007/s00705-002-0785-7.
Feline immunodeficiency virus (FIV) was isolated from five Portuguese cats. The five strains were named RP1, PP2, TLP3, FP4 and CP5. The LTR, the CA region of the gag gene, and the V3-V5 region of the env gene were amplified by PCR, cloned and sequenced. The phylogenetic relationships among these three regions and other previously published sequences revealed an independent clustering of the Portuguese isolates in the LTR and CA protein. Based on the V3-V5 region the Portuguese isolates were classified as Subtype B, although they appear to be a subcluster within Subtype B. The study of these new FIV isolates showed the presence of Subtype B in Portugal and could provide a contribution for the understanding of FIV's genetic diversity.
猫免疫缺陷病毒（FIV）是从五只葡萄牙猫中分离出来的。这五个毒株分别命名为RP1、PP2、TLP3、FP4和CP5。通过聚合酶链反应（PCR）扩增、克隆并测序长末端重复序列（LTR）、gag基因的衣壳（CA）区域以及env基因的V3-V5区域。这三个区域与其他先前发表的序列之间的系统发育关系表明，葡萄牙分离株在LTR和CA蛋白中形成了一个独立的聚类。基于V3-V5区域，葡萄牙分离株被归类为B亚型，尽管它们似乎是B亚型中的一个亚聚类。对这些新的FIV分离株的研究表明葡萄牙存在B亚型，并且可为理解FIV的遗传多样性做出贡献。
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