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从台湾地区猫只分离出的猫免疫缺陷病毒的系统发育分析。

Phylogenetic analysis of feline immunodeficiency virus isolated from cats in Taiwan.

作者信息

Inada G, Miyazawa T, Inoshima Y, Kohmoto M, Ikeda Y, Liu C H, Lin J A, Kuo T F, Mikami T

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

Arch Virol. 1997;142(7):1459-67.

PMID:9267455
Abstract

Feline immunodeficiency virus was isolated from four cats in Taiwan. The isolates were designated TI-1, TI-2, TI-3 and TI-4. Each was isolated from PBMCs following co-cultivation of PBMCs with a feline T-lymphoblastoid cell line (MYA-1 cells). However, the Taiwanese isolates did not grow in a feline kidney cell line (CRFK cells). The nucleotide sequences of the V3-V5 region of the envelope gene of the Taiwanese isolates were determined and compared with those of previously described isolates. Phylogenetic analysis of this region indicates that Taiwanese isolates belong to subtype C.

摘要

从台湾的四只猫身上分离出了猫免疫缺陷病毒。这些分离株被命名为TI-1、TI-2、TI-3和TI-4。每一株都是在将外周血单核细胞(PBMCs)与猫T淋巴母细胞系(MYA-1细胞)共培养后从PBMCs中分离出来的。然而,台湾分离株在猫肾细胞系(CRFK细胞)中不能生长。测定了台湾分离株包膜基因V3-V5区域的核苷酸序列,并与先前描述的分离株进行了比较。该区域的系统发育分析表明,台湾分离株属于C亚型。

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