Foschini Maria Pia, Marucci Gianluca, Eusebi Vincenzo
Department of Oncology, Section of Anatomic Pathology, M. Malpighi University of Bologna, Ospedale Bellaria, Via Altura 3, 40139 Bologna, Italy.
Virchows Arch. 2002 May;440(5):536-42. doi: 10.1007/s00428-001-0585-6. Epub 2002 Feb 9.
The purpose of the present study is to determine the presence and distribution of epithelial and myoepithelial cells in mucoepidermoid carcinoma (MEC) of salivary glands and to compare them with normal salivary gland tissue and other primary carcinomas. This is in order to establish novel diagnostic criteria and to better understand MEC histogenesis. Formalin-fixed paraffin-embedded tissues from ten well-differentiated MECs, three adenoid cystic carcinomas (ACC), four acinic cell carcinomas (AC), and three epithelial-myoepithelial carcinomas (EMCC) of salivary glands were studied with immunohistochemistry using antibodies that recognise antigens indicative of epithelial and myoepithelial cell differentiation. An anti-mitochondrial antibody was also employed. Normal salivary tissue was present for comparative study in non-tumorous areas of the same section from 12 cases. MEC contained numerous keratin-positive cells. Anti mitochondrial antibody was diffusely positive in all ten of these tumours. Smooth muscle actin, h-caldesmon, and smooth muscle heavy chain myosin, which are indicative of myoepithelial cell differentiation, were negative. Rare cells in only one case were stained by calponin. Cytokeratin 14 (CK14) and anti mitochondrial antibody stained cells located mainly at the periphery of neoplastic nests and cystic spaces, while CK7 was mainly present in cells bordering gland lumina (zoning pattern). The immunohistochemical cell profile was similar to that seen in striated normal ducts. All others tumours studied showed a different immunohistochemical pattern, mostly consisting of a lack of mitochondrion-rich cells and the presence of myoepithelial cells in ACC and EMCC. Immunoreactivity in MEC for CK7, CK14 and mitochondrial antibodies appears as a peculiar pattern of staining, different from that of other salivary gland tumors; this seems helpful for diagnostic purposes. In addition, a differentiation of the "striated duct phenotype" is suggested.
本研究的目的是确定涎腺黏液表皮样癌（MEC）中上皮细胞和肌上皮细胞的存在及分布，并将其与正常涎腺组织及其他原发性癌进行比较。这是为了建立新的诊断标准并更好地理解MEC的组织发生。使用识别指示上皮细胞和肌上皮细胞分化抗原的抗体，通过免疫组织化学方法研究了来自10例高分化MEC、3例腺样囊性癌（ACC）、4例腺泡细胞癌（AC）和3例涎腺上皮-肌上皮癌（EMCC）的福尔马林固定石蜡包埋组织。还使用了抗线粒体抗体。在12例相同切片的非肿瘤区域存在正常涎腺组织用于对比研究。MEC含有大量角蛋白阳性细胞。抗线粒体抗体在所有这10个肿瘤中均呈弥漫性阳性。指示肌上皮细胞分化的平滑肌肌动蛋白、h-钙调蛋白和平滑肌重链肌球蛋白均为阴性。仅1例中的罕见细胞被钙结合蛋白染色。细胞角蛋白14（CK14）和抗线粒体抗体染色的细胞主要位于肿瘤巢和囊腔的周边，而CK7主要存在于与腺腔相邻的细胞中（分区模式）。免疫组织化学细胞图谱与正常纹状管中的相似。所有其他研究的肿瘤均显示出不同的免疫组织化学模式，大多表现为缺乏富含线粒体的细胞，且ACC和EMCC中存在肌上皮细胞。MEC中CK7、CK14和线粒体抗体的免疫反应性呈现出一种独特的染色模式，不同于其他涎腺肿瘤；这似乎有助于诊断。此外，提示了“纹状管表型”的分化。
