涎腺黏液表皮样癌和腺样囊性癌中P63、Maspin及基质金属蛋白酶2表达的比较分析
Comparative Analysis of P63, Maspin and Matrix Metalloproteinase 2 Expression in Mucoepidermoid Carcinoma and Adenoid Cystic Carcinoma of Salivary Glands.
作者信息
Taghavi Nasim, Yazdani Farzad, Akbarzadeh Baghban Alireza, Sargolzaei Soudabeh, Kardouni Khoozestani Parisa
机构信息
Dept. of Oral & Maxillofacial Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dept. of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
J Dent (Shiraz). 2020 Jun;21(2):95-101. doi: 10.30476/DENTJODS.2019.77868.0.
STATEMENT OF THE PROBLEM
The tumor suppressor role of myoepithelial cells and related mechanisms in breast tumors are well understood. However, the role of these cells in tumors of salivary glands is debatable.
PURPOSE
The present study was designed to determine the expression of p63, mammary serine protease inhibitor (maspin) and matrix metalloproteinase 2 (MMP-2) in mucoepidermoid carcinoma (MEC) and adenoid cystic carcinoma (ADCC) of salivary glands due to various cellular differentiation and structure. The association between the expression of these markers and clinicopathologic features and myoepithelial differentiation were also evaluated.
MATERIALS AND METHOD
P63, maspin, and MMP-2 expression were immunohistochemically studied in 67 cases including35 cases of MEC and 32 cases of ADCC. The smooth muscle actin (SMA) staining was also applied to confirm the presence of myoepithelial differentiation. Data was analyzed using Chi-square test, Mann-Whitney U test and t-test.
RESULTS
The expression of p63 (= 0.009) and maspin (= 0.012) significantly differed between the study groups. P63 positive cells in MEC were negative for SMA staining in contrast to ADCC. Furthermore, the expression of P63 (= 0.045) and maspin (= 0.019) significantly and inversely correlated with histologic grade in ADCC. Likewise, positive significant correlation was detected between histologic grade and expression of P63 (= 0.018) and MMP-2 (= 0.003) in MEC samples.
CONCLUSION
Our findings showed that MEC is devoid of myoepithelial cells. The difference in expression of P63 and maspin in ADCC and MEC highlighted the role and presence of myoepithelial cells in ADCC. Indeed, the high expression of P63 and maspin in well-differentiated ADCCs suggests the tumor suppressor effect of myoepithelial cells. Considering the association between the evaluated markers and histological grade, p63 in both tumors, maspin in ADCC and MMP-2 in MEC may be efficient predictors of clinical behavior.
问题陈述
肌上皮细胞在乳腺肿瘤中的肿瘤抑制作用及其相关机制已得到充分了解。然而，这些细胞在涎腺肿瘤中的作用仍存在争议。
目的
本研究旨在确定由于各种细胞分化和结构，p63、乳腺丝氨酸蛋白酶抑制剂（maspin）和基质金属蛋白酶2（MMP-2）在涎腺黏液表皮样癌（MEC）和腺样囊性癌（ADCC）中的表达情况。还评估了这些标志物的表达与临床病理特征及肌上皮分化之间的关联。
材料与方法
对67例病例进行免疫组织化学研究，其中包括35例MEC和32例ADCC，检测p63、maspin和MMP-2的表达。同时应用平滑肌肌动蛋白（SMA）染色来确认肌上皮分化的存在。采用卡方检验、曼-惠特尼U检验和t检验进行数据分析。
结果
研究组之间p63（= 0.009）和maspin（= 0.012）的表达存在显著差异。与ADCC相反，MEC中p63阳性细胞的SMA染色为阴性。此外，ADCC中P63（= 0.045）和maspin（= 0.019）的表达与组织学分级显著负相关。同样，在MEC样本中，组织学分级与P63（= 0.018）和MMP-2（= 0.003）的表达之间存在显著正相关。
结论
我们的研究结果表明，MEC中不存在肌上皮细胞。ADCC和MEC中P63和maspin表达的差异突出了肌上皮细胞在ADCC中的作用和存在。事实上，在高分化ADCC中P63和maspin的高表达表明了肌上皮细胞的肿瘤抑制作用。考虑到评估的标志物与组织学分级之间的关联，两种肿瘤中的p63、ADCC中的maspin和MEC中的MMP-2可能是临床行为的有效预测指标。
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