两种碱基高度修饰噬菌体的序列揭示了新的噬菌体-宿主相互作用。

The Sequence of Two Bacteriophages with Hypermodified Bases Reveals Novel Phage-Host Interactions.

机构信息

Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

出版信息

Viruses. 2018 Apr 24;10(5):217. doi: 10.3390/v10050217.

DOI:10.3390/v10050217

PMID:29695085

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5977210/

Abstract

Bacteriophages SP-15 and ΦW-14 are members of the infecting and (formerly ) , respectively. What links them is that in both cases, approximately 50% of the thymine residues are replaced by hypermodified bases. The consequence of this is that the physico-chemical properties of the DNA are radically altered (melting temperature (Tm), buoyant density and susceptibility to restriction endonucleases). Using 454 pyrosequencing technology, we sequenced the genomes of both viruses. Phage ΦW-14 possesses a 157-kb genome (56.3% GC) specifying 236 proteins, while SP-15 is larger at 222 kb (38.6 mol % G + C) and encodes 318 proteins. In both cases, the phages can be considered genomic singletons since they do not possess BLASTn homologs. While no obvious genes were identified as being responsible for the modified base in ΦW-14, SP-15 contains a cluster of genes obviously involved in carbohydrate metabolism.

摘要

噬菌体 SP-15 和 ΦW-14 分别属于和科。它们的共同点是，在这两种情况下，大约 50%的胸腺嘧啶残基被超修饰碱基取代。其结果是，DNA 的理化性质发生了根本改变（熔点（Tm）、浮力密度和对限制内切酶的敏感性）。我们使用 454 焦磷酸测序技术对这两种病毒的基因组进行了测序。噬菌体 ΦW-14 拥有一个 157kb 的基因组（56.3%GC），编码 236 种蛋白质，而 SP-15 更大，为 222kb（38.6mol%G+C），编码 318 种蛋白质。在这两种情况下，噬菌体都可以被认为是基因组的单一拷贝，因为它们没有 BLASTn 同源物。虽然没有明显的基因被确定为 ΦW-14 中修饰碱基的负责者，但 SP-15 包含一组显然与碳水化合物代谢有关的基因簇。