Tebbutt John, Rhodius Virgil A, Webster Christine L, Busby Stephen J W
School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, UK.
FEMS Microbiol Lett. 2002 Apr 23;210(1):55-60. doi: 10.1111/j.1574-6968.2002.tb11159.x.
The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase alpha subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase alpha subunit.
大肠杆菌环磷酸腺苷受体蛋白（CRP）通过与RNA聚合酶α亚基的C末端结构域相互作用，激活靶启动子处的转录。我们构建了一组带有CRP串联DNA位点的启动子，其中一个位点位于-61.5位置，另一个位点位于不同的上游位置。当CRP的上游DNA位点位于-93.5位置或-103.5位置时，观察到最佳的CRP依赖性转录激活。有证据表明，上游结合的CRP分子的激活是由于与RNA聚合酶α亚基的C末端结构域相互作用所致。
