Hu Xuguang, Wen Ya, Liu Shasha, Luo Jiabo, Tan Xiaomei, Li Zhiheng, Lu Xinhua, Long Xiaoying
College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Zhongshuai Pharmaceutical Sci&Tech Incorporated Co., LTD, Zhengzhou, 450000, China.
J Transl Med. 2015 Apr 14;13:121. doi: 10.1186/s12967-015-0478-0.
The anaphylactoid reactions induced by andrographis injection have repeatedly been reported. The aim of our study was to evaluate the immuno-sensitizing potential of extracts from Andrographis paniculata Nees and to screen for the constituent that is responsible for inducing the anaphylactoid reaction.
In the direct popliteal lymph node assay (D-PLNA), female BALB/c mice were randomly divided into several groups with ten mice per group according to the experiment design, the right hind footpads of mice received a single subcutaneous injection of Andrographis paniculata (50 μl), and the left hind footpads received the same volume of vehicle. Seven days later, the mice were sacrificed by cervical dislocation, and the popliteal lymph nodes from both the left and right sides were removed. The weight (WI) and cellularity indices (CI) of the popliteal lymph nodes (PLNs) were then calculated, and the pathological changes of the PLNs were measured. In addition, P815 mast cells were collected for the in vitro cell degranulation experiment. The level of histamine, the percentage of cell degranulation and the ratio of ammonia glycosidase released were measured to further evaluate the potential allergenicity.
Alcohol extract (AEE), ethyl acetate extract (EAE) and n-butanol extract (NBE) significantly increased the weight (WI > 2) and cell number (CI > 5) of PLNs (P < 0.05, P < 0.01). Additionally, all the three monomers of andrographis, namely NAD, AND, and DDA, significantly increased the weight (WI > 2) and cell number (CI > 5) of the PLNs (P < 0.05, P < 0.01). In the cell model, all of the different extract fractions (AEE, EAE and NBE) and the three monomers of andrographis markedly elevated the level of histamine, the percentage of cell degranulation and the ratio of ammonia glycosidase released.
The diterpene lactone compounds of Andrographis paniculata Nees (total lactones of andrographolide) may have a potential sensitizing capacity in andrographis injection.
穿心莲注射液引起的类过敏反应屡有报道。本研究旨在评估穿心莲提取物的免疫致敏潜力，并筛选出引起类过敏反应的成分。
在直接腘窝淋巴结试验（D-PLNA）中，将雌性BALB/c小鼠按实验设计随机分为几组，每组10只。小鼠右后足垫皮下注射穿心莲（50 μl），左后足垫注射相同体积的赋形剂。7天后，通过颈椎脱臼处死小鼠，取出左右两侧的腘窝淋巴结。然后计算腘窝淋巴结（PLN）的重量（WI）和细胞数指数（CI），并检测PLN的病理变化。此外，收集P815肥大细胞进行体外细胞脱颗粒实验。检测组胺水平、细胞脱颗粒百分比和氨糖苷酶释放率，以进一步评估潜在的致敏性。
乙醇提取物（AEE）、乙酸乙酯提取物（EAE）和正丁醇提取物（NBE）显著增加了PLN的重量（WI>2）和细胞数（CI>5）（P<0.05，P<0.01）。此外，穿心莲的三种单体，即NAD、AND和DDA，均显著增加了PLN的重量（WI>2）和细胞数（CI>5）（P<0.05，P<0.01）。在细胞模型中，所有不同的提取物组分（AEE、EAE和NBE）以及穿心莲的三种单体均显著提高了组胺水平、细胞脱颗粒百分比和氨糖苷酶释放率。
穿心莲的二萜内酯化合物（穿心莲内酯总内酯）可能在穿心莲注射液中具有潜在的致敏能力。
