Dusci Leon J, Peter Hackett L, Fellows Linda M, Ilett Kenneth F
Clinical Pharmacology and Toxicology Laboratory, The Western Australian Centre for Pathology and Medical Research, Locked Bag 2009, Nedlands 6009, Australia.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jun 25;773(2):191-7. doi: 10.1016/s1570-0232(02)00164-2.
A rapid method for the determination of olanzapine in plasma using high-performance liquid chromatography with ultra violet detection is described. Olanzapine was extracted from plasma with a mixture of hexane/dichloromethane (85:15), and then back extracted into phosphate buffer pH 2.8. Separation was achieved on a RP Select B C(18) column and commonly administered drugs did not interfere with the assay. The limit of quantitation was 1.5 microg/l and the inter-day and intra-day relative standard deviations were less than 10%. Olanzapine was shown to be stable in plasma for up to 7 days when stored at 4 degrees C. Moreover, the addition of ascorbic acid was not necessary for the achievement of chemical stability during storage, or during the assay procedure. The method has been used to measure olanzapine concentrations in patients treated with various doses of the drug varying from 5 to 40 mg/day.
描述了一种使用高效液相色谱-紫外检测法快速测定血浆中奥氮平的方法。奥氮平用己烷/二氯甲烷（85:15）混合物从血浆中萃取，然后反萃取到pH 2.8的磷酸盐缓冲液中。在RP Select B C(18)柱上实现分离，常用药物不干扰该测定。定量限为1.5微克/升，日间和日内相对标准偏差均小于10%。结果表明，奥氮平在4℃储存时，在血浆中最多可稳定7天。此外，在储存期间或测定过程中，无需添加抗坏血酸来实现化学稳定性。该方法已用于测定接受不同剂量（5至40毫克/天）奥氮平治疗的患者体内的奥氮平浓度。
