Krysan Patrick J, Jester Peter J, Gottwald Jennifer R, Sussman Michael R
Biotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA.
Plant Cell. 2002 May;14(5):1109-20. doi: 10.1105/tpc.001164.
The signal transduction pathways that control cytokinesis in plants are largely uncharacterized. Here, we provide genetic evidence that mitogen-activated protein kinase kinase kinases (MAPKKKs) play a role in the control of plant cell division. Using a reverse-genetic approach, we isolated plants carrying knockout alleles of the Arabidopsis MAPKKK genes ANP1, ANP2, and ANP3. The resulting single-mutant plants displayed no obvious abnormal phenotypes; two of the three double-mutant combinations displayed defects in cell division and growth; and the triple-mutant combination was not transmitted through either male or female gametes. The molecular and structural phenotypes displayed by the double mutants support a model in which the ANP family of MAPKKKs positively regulates cell division and growth and may negatively regulate stress responses.
控制植物胞质分裂的信号转导途径在很大程度上尚未得到充分研究。在这里,我们提供了遗传证据,证明丝裂原活化蛋白激酶激酶激酶(MAPKKKs)在植物细胞分裂的控制中发挥作用。使用反向遗传学方法,我们分离出携带拟南芥MAPKKK基因ANP1、ANP2和ANP3敲除等位基因的植物。所得的单突变体植物没有表现出明显的异常表型;三个双突变体组合中的两个在细胞分裂和生长方面表现出缺陷;而三突变体组合则不能通过雄配子或雌配子传递。双突变体所显示的分子和结构表型支持了一个模型,即MAPKKKs的ANP家族正向调节细胞分裂和生长,并且可能负向调节应激反应。