Levran David, Farhi Jacob, Nahum Hana, Glezerman Marek, Weissman Ariel
In vitro Fertilization Unit, Department of Obstetrics and Gynecology, Wolfson Medical Center, Holon, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Hum Reprod. 2002 Jun;17(6):1604-9. doi: 10.1093/humrep/17.6.1604.
Maturation arrest of human oocytes may occur at various stages of the cell cycle. A total failure of human oocytes to complete meiosis is rarely observed during assisted conception cycles. We describe here a case series of infertile couples for whom all oocytes repeatedly failed to mature during IVF/ICSI. Eight couples, all presenting with unexplained infertility, underwent controlled ovarian stimulation followed by oocyte retrieval and IVF/ICSI. The oocytes were stripped of cumulus cells prior to the ICSI procedure and their maturity status was defined. In each couple, oocyte maturation was repeatedly arrested at the germinal vesicle (GV) (n = 1), metaphase I (MI) (n = 4) and metaphase II (MII) (n = 3) stage. Oocyte maturation arrest may be the cause of infertility in some couples previously classified as having unexplained infertility. The recognition of oocyte maturation arrest as a specific medical condition may contribute to the characterization of the yet poorly defined entity currently known as 'oocyte factor'. The cellular and genetic mechanisms causing oocyte maturation arrest should be the subject of further investigation.
人类卵母细胞的成熟停滞可能发生在细胞周期的各个阶段。在辅助生殖周期中，很少观察到人类卵母细胞完全无法完成减数分裂的情况。我们在此描述一系列不育夫妇的病例，他们在体外受精/卵胞浆内单精子注射（IVF/ICSI）过程中，所有卵母细胞均反复未能成熟。八对夫妇均表现为不明原因的不育，接受了控制性卵巢刺激，随后进行了卵母细胞采集及IVF/ICSI。在ICSI操作前去除卵母细胞的卵丘细胞，并确定其成熟状态。在每对夫妇中，卵母细胞成熟反复停滞在生发泡（GV）期（n = 1）、减数分裂中期I（MI）期（n = 4）和减数分裂中期II（MII）期（n = 3）。卵母细胞成熟停滞可能是一些先前被归类为不明原因不育的夫妇不孕的原因。将卵母细胞成熟停滞识别为一种特定的医学状况，可能有助于对目前定义尚不明确的“卵母细胞因素”这一实体进行特征描述。导致卵母细胞成熟停滞的细胞和遗传机制应作为进一步研究的主题。
