Hodges J K, Czekala N M, Lasley B L
J Med Primatol. 1979;8(6):349-64. doi: 10.1159/000460224.
A practical approach for monitoring urinary estrogen and luteinizing hormone (LH) in various primate species is described. Total immunoreactive estrogen (Et) levels, measured without chromatography, provided a convenient estimation of estrone, estradiol and estriol as the sum of their separated parts. Bioactive LH in urine was measured by the rat interstitial cell testosterone (RICT) in vitro bioassay. Correction for urinary creatinine content allowed for the comparison of hormone production from small volumes of urine collected from single daily voidings. Using these methods, urinary Et and LH profiles through the reproductive cycles of a human, gorilla, chimpanzee and capuchin monkey have been determined.
本文描述了一种监测各种灵长类动物尿液中雌激素和促黄体生成素（LH）的实用方法。未经色谱分离测量的总免疫反应性雌激素（Et）水平，可方便地将雌酮、雌二醇和雌三醇作为其分离部分的总和进行估算。尿液中的生物活性LH通过大鼠间质细胞睾酮（RICT）体外生物测定法进行测量。对尿肌酐含量进行校正后，可比较从每日单次排尿收集的少量尿液中激素的分泌情况。使用这些方法，已确定了人类、大猩猩、黑猩猩和卷尾猴在生殖周期中的尿液Et和LH谱。
