Lasley B L, Hodges J K, Czekala N M
J Reprod Fertil Suppl. 1980;Suppl 28:121-9.
A practical approach for monitoring the reproductive cycle of primates is described. Ovulation, implantation, gestation and post-partum amenorrhoea were identified from measurements of total immunoreactive oestrogen and LH bioactivity in incomplete daily urine samples collected from various species, including a gorilla, a chimpanzee and an orang-utan. Oestrogen values were determined by radioimmunoassay of hydrolysed urine using a non-specific oestriol antiserum and LH was assessed by measuring testosterone output from dispersed rat Leydig cells. Both hormone measurements were indexed by urinary creatinine levels to help adjust for differences in urine concentration and volume.
本文描述了一种监测灵长类动物生殖周期的实用方法。通过测量从包括大猩猩、黑猩猩和猩猩在内的各种物种收集的不完全每日尿液样本中的总免疫反应性雌激素和LH生物活性，来确定排卵、着床、妊娠和产后闭经。雌激素值通过使用非特异性雌三醇抗血清对水解尿液进行放射免疫测定来确定，LH则通过测量分散的大鼠睾丸间质细胞的睾酮产量来评估。两种激素测量均通过尿肌酐水平进行校正，以帮助调整尿液浓度和体积的差异。
