Xie Bo, Zhou Jun-Fu, Lu Qun, Li Chong-Jian, Chen Peng
Huzhou Municipal Central Hospital, 106 Hongqi Road, Huzhou, 313000, Zhejiang, China.
Biomed Environ Sci. 2002 Mar;15(1):48-57.
To study the state of oxidative stress in patients with acute coxsackie virus myocarditis (ACM), and to investigate the pathological chain reactions of a series of free radicals and oxidative and lipoperoxidative damages in their bodies.
Eighty ACM patients and 80 healthy adult volunteers (HAV) were enrolled in a case-control study, in which concentrations of nitric oxide (NO) in plasma, lipoperoxides (LPO) in plasma and LPO in erythrocytes (RBC), vitamin C (VC), vitamin E (VE) and beta-carotene (beta-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in RBC were determined by using spectrophotometric assays.
Compared with the average values (AV) of the above biochemical parameters (BP) in the HAV group, the AV of NO in plasma, and LPO in plasma and RBC in the ACM group were significantly increased (P = 0.0001), while the AV of VC, VE, beta-CAR, SOD, CAT and GSH-Px in the ACM group were significantly decreased (P = 0.0001). The values of the above BP were used to estimate the relative risk ratio (RR) between the ACM group and the HAV group; the RR and its 95% confidence interval were 12.467 (5.745-27.051), 4.333 (2.126-8.834), 6.517 (3.225-13.618), 3.310 (1.598-6.858), 31.000 (12.611-76.201), 4.663 (2.228-9.759), 11.769 (5.440-25.462), 3.043 (1.486-6.229) and 6.594 (3.045-14.281) respectively, and their P levels ranged from 0.002 to 0.0001. The results were as follows: D = 22.143 - 0.017SOD + 0.008NO + 0.244LPO in RBC, Eigenvalue = 13.659, Canonical correlation = 0.965, Wilks' lambda = 0.068, chi 2 = 420.212, P = 0.0001. The correct rate of discrimination to the ACM group and to the HAV group was 87.5% and 95.0%, respectively, and 91.3% of originally grouped cases was correctly classified.
The findings in this study suggested that the oxidative stress in bodies of ACM patients was severely aggravated, and marked high oxidative constituents and low antioxidants and antioxidases in the human body might increase the relative risk of inducing acute coxsackie virus myocarditis, and measuring the values of NO in plasma, SOD and LPO in RBC might increase the correct rates of discriminatory analysis of the ACM.
研究急性柯萨奇病毒心肌炎（ACM）患者的氧化应激状态，探讨其体内一系列自由基及氧化与脂质过氧化损伤的病理连锁反应。
采用病例对照研究，纳入80例ACM患者和80名健康成年志愿者（HAV），用分光光度法测定血浆中一氧化氮（NO）、血浆及红细胞（RBC）中脂质过氧化物（LPO）的浓度，血浆中维生素C（VC）、维生素E（VE）和β-胡萝卜素（β-CAR）的含量，以及RBC中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。
与HAV组上述生化参数（BP）的平均值（AV）相比，ACM组血浆NO、血浆及RBC中LPO的AV显著升高（P = 0.0001），而ACM组VC、VE、β-CAR、SOD、CAT和GSH-Px的AV显著降低（P = 0.0001）。用上述BP值估算ACM组与HAV组之间的相对危险比（RR）；RR及其95%置信区间分别为12.467（5.745 - 27.051）、4.333（2.126 - 8.834）、6.517（3.225 - 13.618）、3.310（1.598 - 6.858）、31.000（12.611 - 76.201）、4.663（2.228 - 9.759）、11.769（5.440 - 25.462）、3.043（1.486 - 6.229）和6.594（3.045 - 14.281），其P值范围为0.002至0.0001。结果如下：RBC中D = 22.143 - 0.017SOD + 0.008NO + 0.244LPO，特征值 = 13.659，典型相关系数 = 0.965，威尔克斯λ = 0.068，卡方 = 420.212，P = 0.0001。对ACM组和HAV组的判别正确率分别为87.5%和95.0%，91.3%的原分组病例被正确分类。
本研究结果提示，ACM患者体内氧化应激严重加剧，人体内显著升高的氧化成分及降低的抗氧化剂和抗氧化酶可能增加诱发急性柯萨奇病毒心肌炎的相对风险，检测血浆NO、RBC中SOD和LPO的值可能提高ACM判别分析的正确率。
