慢性肾小球肾炎患者体内自由基的异常反应及氧化损伤

Abnormal reactions of free radicals and oxidative damages in the bodies of patients with chronic glomerulonephritis.

作者信息

Zhou Jun-Fu, Chen Jia-Xi, Shen Han-Chao, Cai Dong

机构信息

Second Affiliated Hospital, Medical College of Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, China.

出版信息

Biomed Environ Sci. 2002 Sep;15(3):233-44.

PMID:12500664

Abstract

OBJECTIVE

To study the abnormal reactions of a series of free radicals and the oxidative damages induced by free radical abnormal reactions in the bodies of patients with chronic glomerulonephritis.

METHODS

Eighty chronic glomerulonephritis patients (CGNP) and eighty healthy adult volunteers (HAV) were enrolled in a random control study, in which concentrations of nitric oxide (NO) in plasma, lipoperoxides (LPO) in plasma and in erythrocytes, and vitamin C (VC), vitamin E (VE) and beta-carotene (beta-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes were determined with spectrophotometric assays.

RESULTS

Compared with the average values of the above biochemical parameters in the HAV group, the average values of NO in plasma, and LPO in plasma and erythrocytes in the CGNP group were significantly increased (P = 0.0001), while those of VC, VE and beta-CAR in plasma as well as those of SOD, CAT and GPX in erythrocytes in the CGNP group were significantly decreased (P = 0.0001). Pearson product-moment correlation analysis showed that with increase of the concentration of blood creatinine as well as prolongation of the course of disease in the CGNP, the concentrations of NO in plasma, and LPO in plasma and erythrocytes in the CGNP increased gradually, while the concentrations of VC, VE and beta-CAR in plasma as well as the activities of SOD, CAT and GPX in erythrocytes in the CGNP decreased gradually (P = 0.002454-0.000001). The relative risk ratio (RR) of the above biochemical parameters reflecting oxidative damages in the bodies of CGNP ranged from 6.061 to 72.429. The reliability coefficient (alpha) that the above biochemical parameters were used to reflect the oxidative damages of the CGNP was 0.8137, standardized item alpha = 0.9728, Hotelling's T-Squared = 1135680.191, F = 53274.6478, P = 0.000001.

CONCLUSIONS

The findings in this study show that in the bodies of CGNP a series of free radical chain reactions result in severe pathological aggravation and induce oxidative damages in their bodies. Therefore, suitable dose of antioxidants should be supplemented to them so as to alleviate oxidative damages in their bodies.

摘要

目的

研究慢性肾小球肾炎患者体内一系列自由基的异常反应以及自由基异常反应所引发的氧化损伤。

方法

选取80例慢性肾小球肾炎患者（CGNP）和80名健康成年志愿者（HAV）进行随机对照研究，采用分光光度法测定血浆中一氧化氮（NO）、血浆及红细胞中脂质过氧化物（LPO）、血浆中维生素C（VC）、维生素E（VE）和β-胡萝卜素（β-CAR）的浓度，以及红细胞中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性。

结果

与HAV组上述生化参数的平均值相比，CGNP组血浆中NO、血浆及红细胞中LPO的平均值显著升高（P = 0.0001），而CGNP组血浆中VC、VE和β-CAR以及红细胞中SOD、CAT和GPX的平均值显著降低（P = 0.0001）。Pearson积矩相关分析显示，随着CGNP患者血肌酐浓度的升高以及病程的延长，CGNP组血浆中NO、血浆及红细胞中LPO的浓度逐渐升高，而CGNP组血浆中VC、VE和β-CAR的浓度以及红细胞中SOD、CAT和GPX的活性逐渐降低（P = 0.002454 - 0.000001）。上述反映CGNP患者体内氧化损伤的生化参数的相对危险比（RR）范围为6.061至72.4 .29。上述生化参数用于反映CGNP氧化损伤的可靠性系数（α）为0.8137，标准化项目α = 0.9728，Hotelling's T-Squared = 1135680.191，F = 53274.6478，P = 0.000001。