Chang Joon, Kim Nam-Gyun, Piao Zhe, Park Chanil, Park Kang-Sik, Paik Young-Ki, Lee Woo-Jung, Kim Byong Ro, Kim Hoguen
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea.
Cancer Lett. 2002 Aug 28;182(2):193-202. doi: 10.1016/s0304-3835(02)00083-6.
We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.
我们通过比较基因组杂交（CGH）分析检测了22例肝细胞癌（HCC）的染色体变化，并将结果与基于聚合酶链反应的杂合性缺失（PCR-LOH）分析的等位基因分型结果进行了比较。通过CGH分析，在4q（59%）、8p（77%）和16q（50%）染色体区域发现频繁的染色体缺失，而在1q（86%）和8q（77%）发现染色体增加。然而，通过PCR-LOH分析发现所有这些染色体臂都存在频繁的等位基因失衡，9%的染色体畸变仅通过CGH分析检测到，2%仅通过PCR-LOH分析检测到。我们的结果表明，与使用随机选择的微卫星标记进行PCR-LOH分析相比，CGH分析在筛查HCC染色体畸变方面能给出更精确的结果。
