Fu Mao, Chen Hua, Li Xiujun, Li Jie, Wu Bin, Cheng Lihong, Cai Mengyin, Fu Zuzhi
Department of Endocrinology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou, Guangdong, 510120 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Jun;19(3):234-8.
To investigate the association of Pro12Ala variant in peroxisome proliferator-activated receptor-gamma2 gene with type 2 diabetes mellitus and its clinical characteristics.
The genotypes of Pro12Ala variant in peroxisome proliferator-activated receptor-gamma2 gene were determined by polymerase chain reaction-restriction fragment length polymorphisms assay in 401 unrelated subjects of the Han population in the southern part of China (including 180 subjects with normal glucose tolerance and 221 type 2 diabetic patients). The clinical data were also analyzed.
The allele frequencies in the case and control groups were 96.15%,96.11% for P and 3.85%, 3.89% for A; the genotype frequencies were 92.77%, 92.22% for PP, 6.78%, 7.78% for PA and 0.45%, 0 for AA. The Pro12Ala variant of peroxisome proliferator-activated receptor-gamma2 was not significantly associated with type 2 diabetes. The Pro12Ala polymorphism of peroxisome proliferator-activated receptor-gamma2 in diabetes patients was associated with increased waist circumference and waist to hip ratio. The Pro12Ala polymorphism in Chinese population was similar to that in Japanese population and was different from that in European and American population.
The above data showed that the Pro12Ala variant of peroxisome proliferator-activated receptor-gamma2 was not significantly associated with type 2 diabetes, but it could be associated with abdominal obesity in type 2 diabetes. The significant difference of Pro12Ala of peroxisome proliferator-activated receptor-gamma2 among various races was observed.
探讨过氧化物酶体增殖物激活受体γ2基因Pro12Ala变异与2型糖尿病及其临床特征的相关性。
采用聚合酶链反应-限制性片段长度多态性分析法，对中国南方汉族人群401例无亲缘关系的个体（包括180例糖耐量正常者和221例2型糖尿病患者）进行过氧化物酶体增殖物激活受体γ2基因Pro12Ala变异的基因型检测，并分析其临床资料。
病例组和对照组中，P等位基因频率分别为96.15%、96.11%，A等位基因频率分别为3.85%、3.89%；PP基因型频率分别为92.77%、92.22%，PA基因型频率分别为6.78%、7.78%，AA基因型频率分别为0.45%、0。过氧化物酶体增殖物激活受体γ2的Pro12Ala变异与2型糖尿病无显著相关性。糖尿病患者过氧化物酶体增殖物激活受体γ2的Pro12Ala多态性与腰围及腰臀比增加有关。中国人群的Pro12Ala多态性与日本人群相似，与欧美人群不同。
上述数据表明，过氧化物酶体增殖物激活受体γ2的Pro12Ala变异与2型糖尿病无显著相关性，但可能与2型糖尿病患者的腹部肥胖有关。观察到过氧化物酶体增殖物激活受体γ2的Pro12Ala在不同种族间存在显著差异。
