Wang Chuanxian, Zhai Fengying, Chi Yuju, Wang Guichun
National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2004 May;33(3):317-20.
To investigate peroxisome proliferator-activated receptors gamma 2 (PPAR gamma 2) polymorphism and its association with obesity or diabetes in Chinese population.
Normal weight group(91) and overweight and obese group(140) were included in this study, according to BMI. 103 subjects had normal glucose tolerance, 50 had impaired glucose tolerance, and 78 had diabetes mellitus, according to WHO Criteria for Diagnosis of Diabetes Mellitus. We evaluated these subjects for the Pro12Ala mutation in the PPAR gene using PCR-restriction fragment length polymorphism.
Allele frequencies of Pro12Ala mutation of PPAR gamma 2 in overweight and obese subjects (qA = 3.93%) were not significantly different from those in normal weight group (qA = 3.85%, P > 0.05). Both in overweight and obese group and normal weight group, the genotype of PA(AA) had lower plasma resistin as compared with the genotype of PP(P < 0.05). Allele frequencies of Pro12Ala were not different among Chinese subjects with normal glucose tolerance (qA = 3.88%), those with impaired glucose tolerance (qA = 4.00%), and those with diabetes mellitus (qA = 3.85%, P > 0.05). In these three groups, the genotype of PA(AA) had lower plasma resistin as compared with the genotype of PP, only in impaired glucose tolerance group the difference was not significant (P > 0.05).
The Pro12Ala mutation in PPAR gamma 2 was not associated with either diabetes or obesity. The PPAR gamma 2 Ala allele may be involved in the expression of resistin and insulin sensitivity.
研究过氧化物酶体增殖物激活受体γ2（PPARγ2）基因多态性及其与中国人群肥胖或糖尿病的关系。
根据体重指数（BMI）将研究对象分为正常体重组（91例）和超重肥胖组（140例）。根据世界卫生组织糖尿病诊断标准，103例受试者糖耐量正常，50例糖耐量受损，78例患有糖尿病。采用聚合酶链反应-限制性片段长度多态性方法检测PPAR基因的Pro12Ala突变。
超重肥胖组PPARγ2基因Pro12Ala突变的等位基因频率（qA = 3.93%）与正常体重组（qA = 3.85%）相比，差异无统计学意义（P > 0.05）。在超重肥胖组和正常体重组中，PA（AA）基因型的血浆抵抗素水平均低于PP基因型（P < 0.05）。糖耐量正常的中国受试者（qA = 3.88%）、糖耐量受损的受试者（qA = 4.00%）和糖尿病患者（qA = 3.85%）中，Pro12Ala的等位基因频率差异无统计学意义（P > 0.05）。在这三组中，PA（AA）基因型的血浆抵抗素水平均低于PP基因型，仅糖耐量受损组差异无统计学意义（P > 0.05）。
PPARγ2基因的Pro12Ala突变与糖尿病和肥胖均无关联。PPARγ2 Ala等位基因可能参与抵抗素的表达和胰岛素敏感性。
