Kursar Mischo, Bonhagen Kerstin, Köhler Anne, Kamradt Thomas, Kaufmann Stefan H E, Mittrücker Hans-Willi
Max-Planck-Institute for Infection Biology, Berlin, Germany.
J Immunol. 2002 Jun 15;168(12):6382-7. doi: 10.4049/jimmunol.168.12.6382.
The immune response against the intracellular bacterium Listeria monocytogenes involves both CD4(+) and CD8(+) T cells. We used the MHC class II-presented peptide listeriolysin(189-201) to characterize the organ-specific CD4(+) T cell response during infection. Systemic listeriosis resulted in a strong peptide-specific CD4(+) T cell response with frequencies of 1/100 and 1/30 CD4(+) splenocytes at the peak of primary and secondary response, respectively. This response was not restricted to lymphoid organs, because we detected specific CD4(+) T cells in all tissues analyzed. However, the tissue distribution of the T cell response was dependent on the route of infection. After i.v. infection, the strongest CD4(+) T cell response and the highest levels of memory cells were observed in spleen and liver, the major sites of L. monocytogenes replication. After oral infection, we detected a strong response in the liver, the lamina propria, and the intestinal epithelium. These tissues also harbored the highest frequencies of listeriolysin(189-201)-specific CD4(+) memory T cells 5-8 wk post oral infection. Our results show that kinetics and magnitude of the CD4(+) T cell response and the accumulation of CD4(+) memory T cells depend on the route of infection and are regulated in a tissue-specific way.
针对细胞内细菌单核细胞增生李斯特菌的免疫反应涉及CD4(+)和CD8(+) T细胞。我们使用MHC II类呈递的肽李斯特菌溶素（189 - 201）来表征感染期间器官特异性CD4(+) T细胞反应。全身性李斯特菌病导致强烈的肽特异性CD4(+) T细胞反应，在初次和二次反应高峰期，脾细胞中CD4(+) T细胞的频率分别为1/100和1/30。这种反应并不局限于淋巴器官，因为我们在所有分析的组织中都检测到了特异性CD4(+) T细胞。然而，T细胞反应的组织分布取决于感染途径。静脉注射感染后，在单核细胞增生李斯特菌复制的主要部位脾脏和肝脏中观察到最强的CD4(+) T细胞反应和最高水平的记忆细胞。口服感染后，我们在肝脏、固有层和肠上皮中检测到强烈反应。在口服感染后5 - 8周，这些组织中还含有最高频率的李斯特菌溶素（189 - 201）特异性CD4(+)记忆T细胞。我们的结果表明，CD4(+) T细胞反应的动力学和强度以及CD4(+)记忆T细胞的积累取决于感染途径，并以组织特异性方式受到调节。
