Nordengrahn Ann, Merza Malik, Ros Carlos, Lindholmc Arne, Palfl Vilmos, Hannant Duncan, Belák Sándor
Svanova Biotech AB, Uppsala Science Park, Glunten, Sweden.
Vet Res. 2002 May-Jun;33(3):251-9. doi: 10.1051/vetres:2002013.
Equineherpesvirustypes 2 and 5 (EHV-2andEHV-5)have a rather unclearpathogenicity and distribution within the equid population. In order to gain more information on the prevalence of these two viruses, type-specific PCR assays were developed to detect viral DNA in nasal specimens and in peripheral blood leukocytes (PBLs) of adult horses and foals from various regions of Europe, i.e. Sweden, Hungary and the United Kingdom. In adult horses, the prevalence of EHV-2 in PBLs was up to 68% in Sweden and 71% in the United Kingdom. EHV-2 DNA was detected in the PBLs from all the foals tested in all countries and most (93%) of the nasal specimens also yielded positive results. The prevalence of EHV-5 DNA in the PBLs of foals in Hungary was 15 and 24% in adult horses in the United Kingdom. This observation was among the very few reports of the presence of EHV-5 in horses. In summary, the specific PCR assays revealed important data on the occurrence and distribution of EHV-2 and EHV-5 in large horse populations. The findings indicated that infection with EHV-5 occurred later than EHV-2 in foals. This study may contribute to a better understanding of the etiological role of these gammaherpesviruses in equine diseases.
马疱疹病毒2型和5型（EHV - 2和EHV - 5）在马属动物群体中的致病性和分布情况相当不明确。为了获取更多关于这两种病毒流行情况的信息，开发了型特异性PCR检测方法，以检测来自欧洲不同地区（即瑞典、匈牙利和英国）的成年马和幼驹的鼻拭子样本及外周血白细胞（PBL）中的病毒DNA。在成年马中，瑞典PBL中EHV - 2的流行率高达68%，英国为71%。在所有国家检测的所有幼驹的PBL中均检测到EHV - 2 DNA，并且大多数（93%）鼻拭子样本也呈阳性结果。匈牙利幼驹PBL中EHV - 5 DNA的流行率为15%，英国成年马中为24%。这一观察结果是关于马中存在EHV - 5的极少数报道之一。总之，特异性PCR检测方法揭示了EHV - 2和EHV - 5在大型马群中的发生和分布的重要数据。研究结果表明，幼驹感染EHV - 5的时间比EHV - 2晚。这项研究可能有助于更好地理解这些γ疱疹病毒在马疾病中的病因学作用。
