Zou Hanfa, Huang Xiaodong, Ye Mingliang, Luo Quanzhou
National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences.
J Chromatogr A. 2002 Apr 19;954(1-2):5-32. doi: 10.1016/s0021-9673(02)00072-9.
A monolithic stationary phase is the continuous unitary porous structure prepared by in situ polymerization or consolidation inside the column tubing and, if necessary, the surface is functionalized to convert it into a sorbent with the desired chromatographic binding properties [J. Chromatogr. A 855 (1999) 273]. Monolithic stationary phases have attracted considerable attention in liquid chromatography and capillary electrochromatography in recent years due to their simple preparation procedure, unique properties and excellent performance, especially for separation of biopolymers. This review summarizes the preparation, characterization and applications of the monolithic stationary phases. In addition, the disadvantages and limitations of the monolithic stationary phases are also briefly discussed.
整体固定相是通过在柱管内原位聚合或固结制备的连续单一多孔结构，如有必要，对其表面进行功能化处理，使其转化为具有所需色谱结合特性的吸附剂[《色谱杂志A》855 (1999) 273]。近年来，整体固定相因其制备过程简单、性质独特和性能优异，在液相色谱和毛细管电色谱中备受关注，尤其适用于生物聚合物的分离。本文综述了整体固定相的制备、表征及应用。此外，还简要讨论了整体固定相的缺点和局限性。
