肥大细胞在2,4,6-三硝基苯磺酸诱导的大鼠结肠炎发展中的作用。
The role of mast cells in the development of 2, 4, 6-trinitrobenzene sulfonic acid-induced colitis in rats.
作者信息
Fukumoto Y, Kasai H, Takahashi H, Sugiyama H, Hase N, Kaneko H, Hamamura I, Aoki Y, Ota M, Kobayashi T, Katsuura Y, Kamimura T, Komoriya K
机构信息
Pharmacological Research Dept, Teijin Institute for Bio-Medical Research, Asahigaoka, Hino, Tokyo, Japan.
出版信息
Scand J Gastroenterol. 2002 May;37(5):555-60. doi: 10.1080/00365520252903107.
BACKGROUND
The role of mast cells in Crohn disease (CD) remains to be established. The aim of this study was to elucidate this in the development of CD-like colitis in rats by the use of mast-cell-deficient Ws/Ws and their control W+/W+ rats.
METHODS
CD-like colitis was induced in both groups by an enema of 10 mg of 2,4, 6-trinitrobenzene sulfonic acid (TNBS) in 50% ethanol. Colonic damage, adhesion and colonic weight were measured at 7 and 14 days after the TNBS/ethanol enema. Rat mast cell protease-2 (RMCP-2) in the colonic tissue was also measured at 7 days after the enema.
RESULTS
There was no significant difference between W+/W+ and Ws/Ws rats in terms of colonic damage, adhesion or colonic weight. The tissue content of RMCP-2 in Ws/Ws rats treated with either saline or TNBS/ethanol was only maintained at a much lower level than that in W+/W+ rats with the corresponding treatment.
CONCLUSIONS
These results demonstrate that mast cells are not essential in the development of 2, 4, 6-trinitrobenzene sulfonic acid-induced colitis in rats.
背景
肥大细胞在克罗恩病（CD）中的作用仍有待确定。本研究的目的是通过使用肥大细胞缺陷的Ws/Ws大鼠及其对照W+/W+大鼠，在大鼠类CD样结肠炎的发展过程中阐明这一作用。
方法
两组均通过在50%乙醇中灌肠10mg 2,4,6-三硝基苯磺酸（TNBS）诱导类CD样结肠炎。在TNBS/乙醇灌肠后7天和14天测量结肠损伤、粘连和结肠重量。在灌肠后7天也测量结肠组织中的大鼠肥大细胞蛋白酶-2（RMCP-2）。
结果
在结肠损伤、粘连或结肠重量方面，W+/W+和Ws/Ws大鼠之间没有显著差异。用生理盐水或TNBS/乙醇处理的Ws/Ws大鼠中RMCP-2的组织含量仅维持在比相应处理的W+/W+大鼠低得多的水平。
结论
这些结果表明肥大细胞在大鼠2,4,6-三硝基苯磺酸诱导的结肠炎发展中不是必需的。
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