Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Yuzuncu Yil, Van, Turkey.
Eur J Histochem. 2010 Oct 21;54(4):e35. doi: 10.4081/ejh.2010.e35.
This study investigated the effects of royal jelly (RJ) on acetic acid-induced colitis in rats. Twenty adult female Wistar albino rats were divided into four treatment groups of 5 animals each, including a control group (Group I); Group II was treated orally with RJ (150 mg kg(-1) body weight); Group III had acetic acid-induced colitis; and Group IV had acetic acid-induced colitis treated orally with RJ (150 mg kg(-1) body weight) for 4 weeks. Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in all groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with Mallory’s triple stain and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for Mast Cells). RJ was shown to protect the colonic mucosa against the injurious effect of acetic acid. Colitis (colonic damage) was confirmed histomorphometrically as significant increases in the number of mast cells (MC) and colonic erosions in rats with acetic acid-induced colitis. The RJ treatment significantly decreased the number of MC and reduced the area of colonic erosion in the colon of RJ-treated rats compared with rats with untreated colitis. The results suggest that oral treatment with RJ could be used to treat colitis.
本研究旨在探讨蜂王浆（RJ）对乙酸诱导的大鼠结肠炎的影响。将 20 只成年雌性 Wistar 白化大鼠随机分为 4 个治疗组，每组 5 只，包括对照组（I 组）；II 组经口给予 RJ（150mg/kg 体重）；III 组为乙酸诱导的结肠炎；IV 组为乙酸诱导的结肠炎，经口给予 RJ（150mg/kg 体重）治疗 4 周。通过直肠内注入 4%乙酸诱导结肠炎；对照组给予生理盐水（10mL/kg）。所有组别的动物均在深度麻醉下从结肠中取出样本。组织在 10%中性缓冲福尔马林溶液中固定 24 小时，然后包埋在石蜡中。用 Mallory's 三色染色和 1%水溶液中的甲苯胺蓝（pH1.0）染色 6μm 厚的切片 5 分钟（用于肥大细胞）。RJ 被证明可以保护结肠黏膜免受乙酸的损伤作用。结肠炎（结肠损伤）通过组织形态计量学证实，乙酸诱导的结肠炎大鼠的肥大细胞（MC）数量和结肠糜烂显著增加。与未经治疗的结肠炎大鼠相比，RJ 治疗显著减少了 MC 的数量，并减少了 RJ 治疗大鼠结肠的结肠糜烂面积。结果表明，口服 RJ 治疗可能用于治疗结肠炎。
