甘薯（Ipomoea batatas L.）基因型叶片多酚成分的鉴定与表征

Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes.

作者信息

Islam Md Shahidul, Yoshimoto Makoto, Yahara Shoji, Okuno Shigenori, Ishiguro Koji, Yamakawa Osamu

机构信息

Department of Upland Farming Research, National Agricultural Research Center for Kyushu Okinawa Region, Yokoichi 6651-2, Miyakonojo, Miyazaki 885-0091, Japan.

出版信息

J Agric Food Chem. 2002 Jun 19;50(13):3718-22. doi: 10.1021/jf020120l.

DOI:10.1021/jf020120l

PMID:12059149

Abstract

Trials over two years were conducted using 1389 sweetpotato (Ipomoea batatas L.) genotypes collected from all over the world to characterize the polyphenolic composition in sweetpotato leaves. Wide variation was observed in relation to their total and individual leaf polyphenolic constituents. In all genotypes studied, the total polyphenol contents of sweetpotato leaf ranged from 1.42 to 17.1 g/100 g dry weight. The six different polyphenolic compounds were identified and quantified by NMR, FABMS, and RPHPLC analysis procedures. This is the first report of polyphenolic compositions in sweetpotato leaves. The relative levels of polyphenolic acids in sweetpotato leaves were as follows: 3,5-di-O-caffeoylquinic acid > 4,5-di-O-caffeoylquinic acid > chlorogenic acid (3-O-caffeoylquinic acid) > 3,4-di-O-caffeoylquinic acid > 3,4,5-tri-O-caffeoylquinic acid > caffeic acid. The highest 3,4,5-tri-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid occurred at 221 and 1183.30 mg/100 g dry weight, respectively.

摘要

利用从世界各地收集的1389种甘薯（Ipomoea batatas L.）基因型进行了为期两年的试验，以表征甘薯叶片中的多酚成分。观察到其叶片总多酚成分和单个多酚成分存在广泛差异。在所有研究的基因型中，甘薯叶的总多酚含量范围为1.42至17.1克/100克干重。通过核磁共振（NMR）、快原子轰击质谱（FABMS）和反相高效液相色谱（RPHPLC）分析程序鉴定并定量了六种不同的多酚化合物。这是关于甘薯叶片多酚成分的首次报道。甘薯叶中多酚酸的相对含量如下：3,5-二-O-咖啡酰奎尼酸＞4,5-二-O-咖啡酰奎尼酸＞绿原酸（3-O-咖啡酰奎尼酸）＞3,4-二-O-咖啡酰奎尼酸＞3,4,5-三-O-咖啡酰奎尼酸＞咖啡酸。3,4,5-三-O-咖啡酰奎尼酸和4,5-二-O-咖啡酰奎尼酸的最高含量分别为221和1183.30毫克/100克干重。