一种使用发夹形成探针鉴定单核苷酸多态性的电化学检测方案。
An electrochemical detection scheme for identification of single nucleotide polymorphisms using hairpin-forming probes.
作者信息
Huang Tony Jun, Liu Minghsun, Knight Linda D, Grody Wayne W, Miller Jeff F, Ho Chih-Ming
机构信息
Mechanical and Aerospace Engineering Department, University of California-Los Angeles, Los Angeles, CA 90095-1597, USA.
出版信息
Nucleic Acids Res. 2002 Jun 15;30(12):e55. doi: 10.1093/nar/gnf054.
Abstract
Single nucleotide polymorphisms are implicated as having a significant role in regulating growth, development and, thereby, human health and disease. We have developed a method for identifying single nucleotide genetic alterations by combining hairpin-forming DNA probes and electrochemical detection of sandwich DNA hybridization. Incorporation of hairpin-forming competitor probes and the catalyzed reporter deposition amplification system further improves assay specificity by 7-fold and sensitivity by 100-fold. We have demonstrated that the system successfully identified the factor V Leiden mutations from human blood specimens.
摘要
单核苷酸多态性被认为在调节生长、发育以及人类健康和疾病方面具有重要作用。我们开发了一种通过结合形成发夹结构的DNA探针和夹心DNA杂交的电化学检测来识别单核苷酸基因改变的方法。加入形成发夹结构的竞争探针和催化报告沉积扩增系统可进一步将检测特异性提高7倍，灵敏度提高100倍。我们已经证明该系统成功地从人类血液样本中识别出因子V莱顿突变。
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