Brodal A, Walberg F, Hoddevik G H
J Comp Neurol. 1975 Dec 15;164(4):449-69. doi: 10.1002/cne.901640405.
The distribution of labeled cells in the inferior olive of the cat has been mapped following injections of small amounts of horseradish perosidase in the paramedian lobule of the cerebellum. The distribution of labeled cells was plotted in drawings of approximately serial transverse sections. The findings in each case were transferred to a standard diagram of the olive to facilitate comparison of cases. Previous studies of the distribution of retrograde cell loss in the inferior olive following cerebellar lesions (Brodal, '40b) showed that fibers ending in the paramedian lobule come from the caudal part of the ventral lamella of the principla olive. This was confirmed with the peroxidase method, but in addition three other separate and well circumscribed area of the olive showed labeling: one in the dorsal accessory olive, another in the rostral part of the medial accessory olive, a third in the caudal part of the dorsal lamella of the principal olive (fig. 7). There is some degree of topical arrangement within the projection of each of these olivary areas to the paramedian lobule. It is particularly striking that the projection areas of the caudal one-third of the lobule are different from and overlap only little with those of the orstral two-thirds. On account of diffusion of the injected perosidase solution in the folia it could not be decided whether the different olivary areas project to particular longitudinal zones in the paramedian lobule. The main findings can be correlated with the physiological observations of Armstrong et al. ('74). Some of the "paramedian" olivary areas are labeled also following peroxidase injections in other cerebellar parts, among them the nuclei interpositus anterior and posterior. The findings are compatible with the notion that olivocerebellar fibers branch to supply more than one cerebellar region. It is confirmed that the olivocerebellar projection, including that of the nuclei, is almost completely crossed. In the discussion it is emphasized that afferents from several sources converge on all four olivary regions projecting onto the paramedian lobule. The olivocerebellar projection obviously allows for divergence as well as convergence of impulses from the olive to the cerebellum. For further insight into the anatomical organization of the inferior olive, the entire olivocerebellar projection has to be mapped with the peroxidase methods, and further studies of the afferents to the olive are needed. In such studies, as well as in physiological ones, it is essential that findings are described with meticulous reference to the topography of the olivary subdivisions.
在猫的小脑旁正中小叶注射少量辣根过氧化物酶后，已绘制出标记细胞在猫下橄榄核中的分布情况。在大致连续的横切片图中绘制标记细胞的分布。将每个病例的结果转移到橄榄核的标准图上，以便于病例比较。先前关于小脑损伤后下橄榄核逆行性细胞丢失分布的研究（布罗达尔，1940b）表明，终止于旁正中小叶的纤维来自主橄榄核腹侧薄片的尾部。用过氧化物酶法证实了这一点，但此外，橄榄核的其他三个独立且界限分明的区域也显示有标记：一个在背侧副橄榄核，另一个在内侧副橄榄核的前部，第三个在主橄榄核背侧薄片的尾部（图7）。在这些橄榄核区域中，每个区域向旁正中小叶的投射都存在一定程度的局部排列。特别引人注目的是，小叶后三分之一的投射区域与前三分之二的投射区域不同，且仅有少量重叠。由于注射的过氧化物酶溶液在小叶中扩散，无法确定不同的橄榄核区域是否投射到旁正中小叶的特定纵向区域。主要发现可与阿姆斯特朗等人（1974年）的生理学观察结果相关联。在小脑的其他部位，包括前、后间位核注射过氧化物酶后，一些“旁正中”橄榄核区域也有标记。这些发现与橄榄小脑纤维分支以供应多个小脑区域的观点相符。已证实橄榄小脑投射，包括核的投射，几乎完全是交叉的。在讨论中强调，来自多个来源的传入纤维汇聚到投射到旁正中小叶的所有四个橄榄核区域。橄榄小脑投射显然允许冲动从橄榄核发散以及汇聚到小脑。为了进一步深入了解下橄榄核的解剖组织，必须用过氧化物酶法绘制整个橄榄小脑投射图，并且需要对橄榄核的传入纤维进行进一步研究。在这类研究以及生理学研究中，至关重要的是，在描述结果时要仔细参考橄榄核亚区的地形学。
