火鸡马立克氏病。II. 火鸡马立克氏病病毒毒株的病毒糖蛋白B基因及抗原的特性

Marek's disease in turkeys. II. Characterization of the viral glycoprotein B gene and antigen of a turkey strain of Marek's disease virus.

作者信息

Davidson Irit, Malkinson M, Weisman Y

机构信息

Division of Avian and Fish Diseases, Kimron Veterinary Institute, Bet Dagan, Israel.

出版信息

Avian Dis. 2002 Apr-Jun;46(2):322-33. doi: 10.1637/0005-2086(2002)046[0322:MSDITI]2.0.CO;2.

DOI:10.1637/0005-2086(2002)046[0322:MSDITI]2.0.CO;2

PMID:12061641

Abstract

Marek's disease virus (MDV) causes immunosuppression and tumors in chickens. As sporadic cases of Marek's disease (MD) were recorded in turkeys, the antigenic and genomic characteristics of the MDV glycoprotein B (gB) gene and antigen of turkeys were compared to the chicken MDV gB. The whole chicken and turkey gB genes were sequenced and found identical. By immunoblotting of infected-cell culture lysates using chicken convalescent and gB monoclonal antibodies, the antigenic epitopes of the chicken and turkey viruses were found to differ. The turkey MDV had a unique epitope, compared to the chicken MDV and compared with our previous findings. While the chicken MDV had two epitope types, heat-labile but dithiothreitol (DTT)-stable and heat-stable but DTT-labile, the turkey MDV gB epitope is both heat and DTT-labile.

摘要

马立克氏病病毒（MDV）可导致鸡出现免疫抑制和肿瘤。由于火鸡中记录到了散发性马立克氏病（MD）病例，因此将火鸡MDV糖蛋白B（gB）基因和抗原的抗原性及基因组特征与鸡MDV的gB进行了比较。对整个鸡和火鸡的gB基因进行了测序，发现二者相同。通过使用鸡恢复期血清和gB单克隆抗体对感染细胞培养物裂解物进行免疫印迹分析，发现鸡和火鸡病毒的抗原表位有所不同。与鸡MDV以及我们之前的研究结果相比，火鸡MDV具有一个独特的表位。鸡MDV有两种表位类型，即热不稳定但二硫苏糖醇（DTT）稳定型以及热稳定但DTT不稳定型，而火鸡MDV的gB表位则对热和DTT均不稳定。