Davidson Irit, Malkinson M, Weisman Y
Division of Avian and Fish Diseases, Kimron Veterinary Institute, Bet Dagan, Israel.
Avian Dis. 2002 Apr-Jun;46(2):322-33. doi: 10.1637/0005-2086(2002)046[0322:MSDITI]2.0.CO;2.
Marek's disease virus (MDV) causes immunosuppression and tumors in chickens. As sporadic cases of Marek's disease (MD) were recorded in turkeys, the antigenic and genomic characteristics of the MDV glycoprotein B (gB) gene and antigen of turkeys were compared to the chicken MDV gB. The whole chicken and turkey gB genes were sequenced and found identical. By immunoblotting of infected-cell culture lysates using chicken convalescent and gB monoclonal antibodies, the antigenic epitopes of the chicken and turkey viruses were found to differ. The turkey MDV had a unique epitope, compared to the chicken MDV and compared with our previous findings. While the chicken MDV had two epitope types, heat-labile but dithiothreitol (DTT)-stable and heat-stable but DTT-labile, the turkey MDV gB epitope is both heat and DTT-labile.
马立克氏病病毒（MDV）可导致鸡出现免疫抑制和肿瘤。由于火鸡中记录到了散发性马立克氏病（MD）病例，因此将火鸡MDV糖蛋白B（gB）基因和抗原的抗原性及基因组特征与鸡MDV的gB进行了比较。对整个鸡和火鸡的gB基因进行了测序，发现二者相同。通过使用鸡恢复期血清和gB单克隆抗体对感染细胞培养物裂解物进行免疫印迹分析，发现鸡和火鸡病毒的抗原表位有所不同。与鸡MDV以及我们之前的研究结果相比，火鸡MDV具有一个独特的表位。鸡MDV有两种表位类型，即热不稳定但二硫苏糖醇（DTT）稳定型以及热稳定但DTT不稳定型，而火鸡MDV的gB表位则对热和DTT均不稳定。
