Hassanin Ola, Abdallah Fatma, El-Araby Iman E
ADepartment of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt, 445119.
Avian Dis. 2013 Jun;57(2 Suppl):555-61. doi: 10.1637/10337-082912-Reg.1.
In spite of the intensive vaccination policy against the Marek's disease virus (MDV) in Egypt, the Egyptian poultry flocks are still suffering from several tumor and paralysis cases. To investigate if MDV is the possible cause, feather follicle and tumor samples were collected during 2011 from 30 vaccinated chicken flocks experiencing nervous signs, emaciation, and tumor lesions. The samples were screened by PCR amplification of the meq full-length gene. Only five of 30 flocks were positive for MDV. Additionally, we sequenced meq from five samples and gL and gC from three samples. A phylogenetic tree was constructed using the deduced amino acid sequences of the meq gene. The sequence analysis revealed that most of the studied sequences showed > or = 98% identity to the very virulent European ATE and C12/130 isolates and the very virulent Chinese LMS, YA, WS03, and GX070060 MDV isolates. The two glycoproteins, gL and gC, displayed high similarity to the classical MDV strains published in the database regardless of their virulence.
尽管埃及实施了针对马立克氏病病毒（MDV）的强化疫苗接种政策，但埃及的家禽群仍遭受多起肿瘤和麻痹病例。为调查MDV是否为可能的病因，2011年期间从30个出现神经症状、消瘦和肿瘤病变的接种鸡群中采集了毛囊和肿瘤样本。通过对meq全长基因进行PCR扩增来筛选样本。30个鸡群中只有5个MDV呈阳性。此外，我们对5个样本的meq以及3个样本的gL和gC进行了测序。使用meq基因推导的氨基酸序列构建了系统发育树。序列分析表明，大多数研究序列与超强毒力的欧洲ATE和C12/130分离株以及超强毒力的中国LMS、YA、WS03和GX070060 MDV分离株显示出≥98%的同一性。两种糖蛋白gL和gC，无论其毒力如何，与数据库中公布的经典MDV毒株都具有高度相似性。
