Pawlak Anna, Rózanowska Małgorzata, Zareba Mariusz, Lamb Laura E, Simon John D, Sarna Tadeusz
Department of Biophysics, Jan Zurzycki Institute of Molecular Biology and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Arch Biochem Biophys. 2002 Jul 1;403(1):59-62. doi: 10.1016/S0003-9861(02)00260-6.
The action spectra for the photoconsumption of oxygen by lipofuscin isolated from human retinal pigment epithelium cells and liposomal suspensions containing extracts of lipofuscin are reported. The lipofuscin and lipofuscin extract action spectra are similar, demonstrating the phototoxic constituents of lipofuscin are present in the lipofuscin solvent extract. 2-[2,6-Dimethyl-8-(2,6,6-trimethyl-1-cyclohexen-1-yl)-1E,3E,5E,7E-octatetraenyl]-1-(2-hydroxyethyl)-4-[4-methyl-6-(2,6,6-trimethyl-1-cyclohexen-1-yl)-1E,3E,5E-hexatrienyl]-pyridinium (A2E), present in both intact granules and the solvent extract, has been invoked as an important contributor to the phototoxicity of lipofuscin. The action spectrum for oxygen photoconsumption by A2E follows its absorption spectrum but does not resemble the action spectrum for photoconsumption of oxygen by lipofuscin granules or lipofuscin extract. These results combined with recently reported experimental studies on the aerobic photoreactivity of A2E indicate that it is not a major contributor to the phototoxicity of lipofuscin.
报道了从人视网膜色素上皮细胞分离的脂褐素以及含有脂褐素提取物的脂质体悬浮液光消耗氧气的作用光谱。脂褐素和脂褐素提取物的作用光谱相似，表明脂褐素的光毒性成分存在于脂褐素的溶剂提取物中。完整颗粒和溶剂提取物中均存在的2-[2,6-二甲基-8-(2,6,6-三甲基-1-环己烯-1-基)-1E,3E,5E,7E-辛四烯基]-1-(2-羟乙基)-4-[4-甲基-6-(2,6,6-三甲基-1-环己烯-1-基)-1E,3E,5E-己三烯基]-吡啶鎓（A2E）被认为是脂褐素光毒性的重要促成因素。A2E光消耗氧气的作用光谱与其吸收光谱一致，但与脂褐素颗粒或脂褐素提取物光消耗氧气的作用光谱不同。这些结果与最近报道的关于A2E有氧光反应性的实验研究相结合，表明它不是脂褐素光毒性的主要促成因素。
