Matar G M, Abdo D, Khneisser I, Youssef M, Zouheiry H, Abdelnour G, Harakeh H S
Department of Microbiology and Immunology, American University of Beirut, P.O. Box 11-0236, Riad El-Solh, Lebanon.
Ann Trop Med Parasitol. 2002 Apr;96(3):317-24. doi: 10.1179/000349802125001032.
In several hospitals in Beirut, Lebanon, 77 isolates of Escherichia coli were successfully derived from the stools of patients with diarrhoeal diseases, by culture on MacConkey or MacConkey-sorbitol agar. When the isolates were screened, using a multiplex PCR, 14 (from 14 different patients) were each found positive for one of the various genes defining the enterotoxigenic (five), enteroinvasive (four), enteroaggregative (three) or enteropathogenic (two) groups. Genotyping of these 14 diarrhoeagenic isolates, by pulsed-field gel electrophoresis, indicated that all were genomically distinct with the exception of two of the enteroaggregative isolates (which were of the same genotype). The E. coli apparently involved in diarrhoeal disease in Beirut therefore belong to at least four different diarrhoeagenic groups and show strain variation within each group. Diarrhoea in the absence of diarrhoeagenic E. coli may be the result of infection with bacteria other than E. coli or viral or parasitic enteropathogens.
在黎巴嫩贝鲁特的几家医院，通过在麦康凯或麦康凯 - 山梨醇琼脂上培养，从腹泻病患者的粪便中成功分离出77株大肠杆菌。当使用多重聚合酶链反应（PCR）对分离株进行筛查时，发现14株（来自14名不同患者）分别对定义产肠毒素性（5株）、侵袭性（4株）、聚集性（3株）或致病性（2株）组的各种基因中的一种呈阳性。通过脉冲场凝胶电泳对这14株致腹泻分离株进行基因分型，结果表明，除了两株聚集性分离株（属于相同基因型）外，所有分离株在基因组上都是不同的。因此，贝鲁特明显参与腹泻病的大肠杆菌至少属于四个不同的致腹泻组，并且每组内都存在菌株变异。没有产腹泻性大肠杆菌时的腹泻可能是由大肠杆菌以外的细菌或病毒或寄生性肠道病原体感染引起的。
