Saleem Kadharbatcha S, Pauls Jon M, Augath Mark, Trinath Torsten, Prause Burkhard A, Hashikawa Tsutomu, Logothetis Nikos K
Riken Brain Science Institute, 2-1 Hirosawa, Wakoshi, Saitama 351-0198, Japan.
Neuron. 2002 May 30;34(5):685-700. doi: 10.1016/s0896-6273(02)00718-3.
Recently, an MRI-detectable, neuronal tract-tracing method in living animals was introduced that exploits the anterograde transport of manganese (Mn2+). We present the results of experiments simultaneously tracing manganese chloride and wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) to evaluate the specificity of the former by tracing the neuronal connections of the basal ganglia of the monkey. Mn2+ and WGA-HRP yielded remarkably similar and highly specific projection patterns. By showing the sequential transport of Mn2+ from striatum to pallidum-substantia nigra and then to thalamus, we demonstrated MRI visualization of transport across at least one synapse in the CNS of the primate. Transsynaptic tract tracing in living primates will allow chronic studies of development and plasticity and provide valuable anatomical information for fMRI and electrophysiological experiments in primates.
最近，一种可通过MRI检测的活体动物神经元示踪方法被引入，该方法利用了锰（Mn2+）的顺行运输。我们展示了同时追踪氯化锰和与辣根过氧化物酶偶联的小麦胚凝集素（WGA-HRP）的实验结果，通过追踪猴子基底神经节的神经元连接来评估前者的特异性。Mn2+和WGA-HRP产生了非常相似且高度特异的投射模式。通过展示Mn2+从纹状体到苍白球-黑质再到丘脑的顺序运输，我们证明了在灵长类动物中枢神经系统中至少跨越一个突触的运输的MRI可视化。在活体灵长类动物中进行跨突触示踪将允许对发育和可塑性进行长期研究，并为灵长类动物的功能磁共振成像（fMRI）和电生理实验提供有价值的解剖学信息。
