Kusaka Takashi, Isobe Kenichi, Nagano Keiko, Okubo Kensuke, Yasuda Saneyuki, Kondo Masatoshi, Itoh Susumu, Hirao Konomu, Onishi Shoju
Maternal and Children's Medical Center, Kagawa Medical University, 1750-1 Mikicho, Kitagun, Kagawa 761-0793, Japan.
Comp Biochem Physiol A Mol Integr Physiol. 2002 May;132(1):121-32. doi: 10.1016/s1095-6433(01)00538-4.
The aim of this study was to quantify the relative concentrations of oxyhemoglobin and deoxyhemoglobin within the light path of the brain and to estimate cerebral hemoglobin (Hb) oxygen saturation using full-spectrum near-infrared spectroscopy (fsNIRS). For this purpose, we developed a novel exponential correction equation as well as a two-point spectroscopy method to estimate the relative concentrations of Hb and Hb oxygen saturation in biological tissues. The results of evaluation of measurements using an in vitro model indicated that our fsNIRS method enables accurate and non-invasive measurements of Hb content and saturation in a highly scattered medium such as the human brain. According to the results of analysis using a hypoxic piglet model, the mean cerebral Hb oxygen saturation (SbO(2)) of newborn piglets at an inspired oxygen gas concentration of 0.21 was estimated to be 63+/-4% (mean+/-S.D.). Umbilical arterial and left internal jugular venous Hb oxygen saturation were simultaneously estimated to be 96+/-2% and 52+/-11%, respectively. SbO(2) and arterial Hb oxygen saturation values had a linear relationship. The average oxygenation state of cerebral tissue is comparable with that of the cerebral vein. The results of this study showed that our method can be used to monitor Hb oxygen saturation in the neonatal brain at the bedside in an intensive care unit.
本研究的目的是量化大脑光程内氧合血红蛋白和脱氧血红蛋白的相对浓度，并使用全光谱近红外光谱法（fsNIRS）估算脑血红蛋白（Hb）氧饱和度。为此，我们开发了一种新型指数校正方程以及两点光谱法，以估算生物组织中Hb的相对浓度和Hb氧饱和度。使用体外模型进行测量评估的结果表明，我们的fsNIRS方法能够在诸如人脑这样的高度散射介质中准确、无创地测量Hb含量和饱和度。根据使用缺氧仔猪模型进行分析的结果，吸入氧气浓度为0.21时新生仔猪的平均脑Hb氧饱和度（SbO₂）估计为63±4%（平均值±标准差）。同时，脐动脉和左颈内静脉Hb氧饱和度分别估计为96±2%和52±11%。SbO₂与动脉Hb氧饱和度值呈线性关系。脑组织的平均氧合状态与脑静脉的相当。本研究结果表明，我们的方法可用于重症监护病房床边监测新生儿脑内的Hb氧饱和度。
