Quertemont Etienne, Linotte Sylvie, de Witte Philippe
Biologie du Comportement, Université catholique de Louvain, Place Croix du Sud 1, 1348 Louvain-La-Neuve, Belgium.
Eur J Pharmacol. 2002 May 31;444(3):143-50. doi: 10.1016/s0014-2999(02)01648-5.
Several microdialysis studies have investigated the effects of acute ethanol on extracellular amino acids in various rat brain regions. However, these studies led to conflicting results, suggesting that individual differences between rat strains and lines may play an important role. In the present study, high-alcohol sensitive (HAS) and low-alcohol sensitive (LAS) rats were used to investigate the possible relationship between ethanol sensitivity and the concentrations of extracellular amino acids in the nucleus accumbens. Several groups of HAS and LAS rats were injected with either saline or ethanol (1.0, 2.0 or 3.0 g/kg, i.p.) and the concentrations of amino acids in the nucleus accumbens microdialysates were assayed by electrochemical detection. Acute ethanol induced a dose-dependent increase in extracellular taurine concentrations. However, this increase was significantly reduced at 2.0 and 3.0 g/kg ethanol in HAS rats relative to LAS rats. Since the biological functions of taurine suggest its implication in the reduction of ethanol adverse effects, a higher increase in taurine concentrations may contribute to the lower ethanol sensitivity of LAS rats. Although 2.0 and 3.0 g/kg ethanol did not affect extracellular glutamate concentrations, a significant increase in glutamate was observed after 1.0 g/kg ethanol to HAS rats but not to LAS rats. Such an effect remains unexplained but suggests that discrepancies between the results of previous microdialysate studies may be related to differences in the ethanol sensitivities of various rat strains.
多项微透析研究调查了急性乙醇对大鼠不同脑区细胞外氨基酸的影响。然而，这些研究结果相互矛盾，表明大鼠品系和株系之间的个体差异可能起重要作用。在本研究中，使用高酒精敏感性（HAS）和低酒精敏感性（LAS）大鼠来研究乙醇敏感性与伏隔核中细胞外氨基酸浓度之间的可能关系。给几组HAS和LAS大鼠注射生理盐水或乙醇（1.0、2.0或3.0 g/kg，腹腔注射），并通过电化学检测法测定伏隔核微透析液中氨基酸的浓度。急性乙醇诱导细胞外牛磺酸浓度呈剂量依赖性增加。然而，相对于LAS大鼠，2.0和3.0 g/kg乙醇剂量下HAS大鼠的这种增加显著减少。由于牛磺酸的生物学功能表明其与减轻乙醇不良反应有关，牛磺酸浓度较高的增加可能导致LAS大鼠乙醇敏感性较低。虽然2.0和3.0 g/kg乙醇不影响细胞外谷氨酸浓度，但在1.0 g/kg乙醇处理后，HAS大鼠出现谷氨酸显著增加，而LAS大鼠未出现。这种效应尚无法解释，但表明先前微透析研究结果的差异可能与不同大鼠品系乙醇敏感性的差异有关。
