Aattour Najat, Bouras Mohammed, Tome Daniel, Marcos Ascension, Lemonnier Daniel
INRA, Laboratoire de Nutrition Humaine et de Physiologie Intestinale, Institut National Agronomique Paris-Grignon, France.
Br J Nutr. 2002 Apr;87(4):367-73. doi: 10.1079/bjnbjn2001527.
The effect of feeding lactic-acid bacteria on indices of functions of lymphocytes obtained from Peyer's patches, peripheral blood and spleen from inbred Wistar-Furth rats were studied. Rats were fed on purified diets supplemented with 350 g milk or yoghurt/kg diet for 4 weeks. At the end of the feeding period, immune cells from the three sites were isolated and proliferation, interferon-gamma production and lymphocyte subset composition were studied. Rats consuming yoghurt had a greater in vitro proliferative response to yoghurt bacteria in the three lymphoid compartments, a greater interferon-gamma production in response to bacteria and concanavalin A in Peyer's patches and spleen, and a greater number of Peyer's patches B lymphocytes than milk-fed rats. Macrophage and T lymphocyte proportions and lymphocyte subset composition in the three sites were unaffected by yoghurt. These results indicate that feeding live bacteria contained in yoghurt may interact with the intestinal immune system, and influence the systemic immune system.
研究了给近交系Wistar-Furth大鼠喂食乳酸菌对取自派尔集合淋巴结、外周血和脾脏的淋巴细胞功能指标的影响。大鼠喂食添加了350 g牛奶或酸奶/千克饲料的纯化饲料4周。在喂食期结束时，分离出三个部位的免疫细胞，并研究其增殖、γ-干扰素产生和淋巴细胞亚群组成。食用酸奶的大鼠在三个淋巴区室对酸奶细菌的体外增殖反应更强，在派尔集合淋巴结和脾脏中对细菌和伴刀豆球蛋白A的γ-干扰素产生更多，且派尔集合淋巴结B淋巴细胞数量比喂食牛奶的大鼠更多。酸奶对三个部位的巨噬细胞和T淋巴细胞比例以及淋巴细胞亚群组成没有影响。这些结果表明，喂食酸奶中所含的活菌可能与肠道免疫系统相互作用，并影响全身免疫系统。
