Wang Chau Jong, Hsieh Yu Jin, Chu Chia Yih, Lin Yu Ling, Tseng Tsui Hwa
Institute of Biochemistry, Chung Shan Medical University, Taichung, Taiwan.
Cancer Lett. 2002 Sep 26;183(2):163-8. doi: 10.1016/s0304-3835(02)00031-9.
Esculetin, a coumarin compound, was found to inhibit cell growth and cell cycle progression by inducing arrest of the G1 phase in HL-60 cells. To obtain information regarding cell cycle arrest induced by esculetin, we examined its effect on the regulating factors of the G1 phase in the leukemia HL-60 cells treated with esculetin by Western blotting. Our observations were: (1) a distinct increase in the level of hypophosphorylated retinoblastoma protein (pRb), and a reduction in the level of CDK4 after treatment with 100 microM of esculetin for 24 h; (2) a marked up-regulation of p27, and a down-regulation of cyclin D1 after treatment with 100 microM esculetin for 24 h. These results suggest that esculetin can inhibit the growth of human leukemia HL-60 cells by G1 phase cell cycle arrest as a result of inhibited pRb phosphorylation.
七叶亭，一种香豆素化合物，被发现可通过诱导HL-60细胞G1期停滞来抑制细胞生长和细胞周期进程。为了获取有关七叶亭诱导细胞周期停滞的信息，我们通过蛋白质印迹法检测了七叶亭处理的白血病HL-60细胞中七叶亭对G1期调节因子的影响。我们的观察结果如下：（1）用100微摩尔/升七叶亭处理24小时后，低磷酸化视网膜母细胞瘤蛋白（pRb）水平明显升高，CDK4水平降低；（2）用100微摩尔/升七叶亭处理24小时后，p27显著上调，细胞周期蛋白D1下调。这些结果表明，七叶亭可通过抑制pRb磷酸化导致G1期细胞周期停滞，从而抑制人白血病HL-60细胞的生长。
