Dacheux Denis, Epaulard Olivier, de Groot Arjan, Guery Benoit, Leberre Rozen, Attree Ina, Polack Benoit, Toussaint Bertrand
Département de Biologie Moléculaire et Structurale, BBSI, UMR 5092 CNRS, CEA-Grenoble, CHU-Grenoble, Grenoble, France.
Infect Immun. 2002 Jul;70(7):3973-7. doi: 10.1128/IAI.70.7.3973-3977.2002.
Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.
铜绿假单胞菌临床囊性纤维化分离株CHA用Tn5Tc诱变,以鉴定参与III型分泌系统(TTSS)依赖性对人多形核中性粒细胞细胞毒性的新基因。在25个TTSS功能受影响的突变体中,14个在编码丙酮酸脱氢酶的PDH-E1和-E2亚基的aceAB操纵子的不同位置含有插入。在PDH突变体中,未发生TTSS基因对钙耗竭的转录激活。ExsA的反式表达恢复了TTSS功能和细胞毒性。