Joynson J A, Forbes B, Lambert R J W
Kings College London, Sharnbrook, Bedfordshire, UK.
J Appl Microbiol. 2002;93(1):96-107. doi: 10.1046/j.1365-2672.2002.01667.x.
To produce strains of antimicrobial-resistant Pseudomonas aeruginosa via adaptation to benzalkonium chloride, amikacin and tobramycin and to then examine the incidence, or otherwise, of cross-resistance between antibiotics and between antibiotics and benzalkonium chloride.
Adaptation was obtained by progressive subculturing in subinhibitory concentrations of the antimicrobials. Pseudomonas aeruginosa NCIMB 10421 adapted to grow in high concentrations of benzalkonium chloride (BC) had lower MIC to antibiotics than the wild type, whereas Ps. aeruginosa adapted to grow in antibiotics had greater MIC to benzalkonium by a small degree.
Adaptive resistance to BC of Ps. aeruginosa generally produced cultures with a decrease in resistance to several antibiotics. Adaptive resistance to the aminoglycosides Ak and Tm produced a low-level increase in tolerance to BC. The adaptive mechanisms of resistance appear to be different for the different types of antimicrobials used.
The relationships between biocide and antibiotic resistance are complex. It appears, from this study, that an organism resistant to a common biocide can become sensitive to antibiotics, but the converse was not true. Could this observation be used in a strategy to alleviate antibiotic resistance?
通过让铜绿假单胞菌适应苯扎氯铵、阿米卡星和妥布霉素来产生耐药菌株，然后检测抗生素之间以及抗生素与苯扎氯铵之间交叉耐药的发生率。
通过在亚抑菌浓度的抗菌剂中逐步传代培养来实现适应。适应在高浓度苯扎氯铵（BC）中生长的铜绿假单胞菌NCIMB 10421对抗生素的最低抑菌浓度（MIC）低于野生型，而适应在抗生素中生长的铜绿假单胞菌对苯扎氯铵的MIC略有升高。
铜绿假单胞菌对BC的适应性耐药通常会使培养物对几种抗生素的耐药性降低。对氨基糖苷类药物阿米卡星（Ak）和妥布霉素（Tm）的适应性耐药会使对BC的耐受性有低水平的增加。对于所使用的不同类型抗菌剂，耐药的适应机制似乎有所不同。
杀菌剂与抗生素耐药性之间的关系很复杂。从这项研究来看，似乎对一种常见杀菌剂耐药的生物体可能会变得对抗生素敏感，但反之则不然。这一观察结果能否用于缓解抗生素耐药性的策略中呢？
