Jander Georg, Norris Susan R, Rounsley Steven D, Bush David F, Levin Irena M, Last Robert L
Cereon Genomics LLC, 45 Sidney Street, Cambridge, MA 02139, USA.
Plant Physiol. 2002 Jun;129(2):440-50. doi: 10.1104/pp.003533.
Map-based cloning is an iterative approach that identifies the underlying genetic cause of a mutant phenotype. The major strength of this approach is the ability to tap into a nearly unlimited resource of natural and induced genetic variation without prior assumptions or knowledge of specific genes. One begins with an interesting mutant and allows plant biology to reveal what gene or genes are involved. Three major advances in the past 2 years have made map-based cloning in Arabidopsis fairly routine: sequencing of the Arabidopsis genome, the availability of more than 50,000 markers in the Cereon Arabidopsis Polymorphism Collection, and improvements in the methods used for detecting DNA polymorphisms. Here, we describe the Cereon Collection and show how it can be used in a generic approach to mutation mapping in Arabidopsis. We present the map-based cloning of the VTC2 gene as a specific example of this approach.
基于图谱的克隆是一种迭代方法，用于确定突变表型的潜在遗传原因。这种方法的主要优势在于能够利用几乎无限的自然和诱导遗传变异资源，而无需事先假设或了解特定基因。研究人员从一个有趣的突变体入手，让植物生物学揭示涉及哪些基因。过去两年的三项重大进展使拟南芥中的基于图谱的克隆变得相当常规：拟南芥基因组测序、Cereon拟南芥多态性文库中超过50000个标记的可用性以及用于检测DNA多态性的方法的改进。在这里，我们描述了Cereon文库，并展示了如何将其用于拟南芥突变体定位的通用方法。我们以VTC2基因的基于图谱的克隆作为该方法的一个具体实例。
