Liu T L, Kuai B K, Liu Z X, Zhao D L, Shen D L
State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, China.
Genetika. 2006 Aug;42(8):1089-95.
In Arabidopsis, map-based cloning has been developed to an effective method in mutant genetic analysis because high-density markers are available, candidate genes or genomic sequences can be amplified by PCR and transgenic techniques are simplified. Mutant ses named from shortened early-stage siliques was used as an example to show how to map a mutant in this day. By the process of bulked segregants analysis, linkage testing, large-scale and fine scale mapping, mutant ses was narrowed into a 67 kb interval from CER448792 (2000541 bp) to CER464544 (2067844 bp) crossing over the right of BAC F12K11 to the left of the BAC F4H5 including at most 22 putative genes on the top of chromosome l. In sequence-based map of Arabidopsis genes with Mutant phenotype (SMAGMP) mutant ses was between ATlg06150 (EMB1444) and ATlg08060 (MOM). The SES mapping also showed that developed markers on polymorphism site of CAPC not only were simplified and but worked well. 24 markers from CAPC used in the mapping maybe help Arabidopsis researches with others and the methods related to SES mapping also gave an example of positional cloning.
在拟南芥中，基于图谱的克隆已发展成为突变体遗传分析中的一种有效方法，因为有高密度标记可用，候选基因或基因组序列可通过PCR扩增，并且转基因技术也得到了简化。以从早期缩短的角果命名的突变体ses为例，展示了当今如何定位突变体。通过混合分离群体分析、连锁测试、大规模和精细定位过程，突变体ses被定位到一个67 kb的区间，从CER448792（2000541 bp）到CER464544（2067844 bp），跨越BAC F12K11右侧到BAC F4H5左侧，在第1号染色体顶部最多包含22个推定基因。在基于序列的拟南芥基因与突变体表型图谱（SMAGMP）中，突变体ses位于ATlg06150（EMB1444）和ATlg08060（MOM）之间。SES定位还表明，在CAPC多态性位点上开发的标记不仅简化而且效果良好。在定位中使用的24个来自CAPC的标记可能有助于其他拟南芥研究，并且与SES定位相关的方法也给出了一个位置克隆的例子。
