Nardin Corinne, Meier Wolfgang
Institut für Physikalische Chemie, Universität Basel, Switzerland.
J Biotechnol. 2002 Mar;90(1):17-26. doi: 10.1016/s1389-0352(01)00052-6.
Self-assembly of reactive amphiphilic block copolymers is used to prepare nanostructured hydrogels with exceptional permeability properties, vesicular structures and planar, freestanding membranes in aqueous solution. Although the underlying block copolymer membranes are two-three-fold thicker than conventional lipid bilayers, they can be regarded as mimetic of biological membranes and can be used as a matrix for membrane-spanning proteins. Surprisingly, the proteins remain functional, despite the extreme thickness of the membranes and even after polymerization of the reactive block copolymers. The unique combination of block copolymers with membrane proteins allows the preparation of mechanically stable, defect-free membranes and nanocapsules that have highly selective permeability and/or specific recognition sites. This is documented by some representative examples.
反应性两亲性嵌段共聚物的自组装用于在水溶液中制备具有优异渗透性、囊泡结构以及平面独立膜的纳米结构水凝胶。尽管底层的嵌段共聚物膜比传统脂质双层厚两到三倍，但它们可被视为生物膜的模拟物，并可用作跨膜蛋白的基质。令人惊讶的是，尽管膜的厚度极大，甚至在反应性嵌段共聚物聚合后，蛋白质仍保持功能。嵌段共聚物与膜蛋白的独特组合使得能够制备出具有高选择性渗透性和/或特异性识别位点的机械稳定、无缺陷的膜和纳米胶囊。一些代表性例子证明了这一点。
