Ross David, Locascio Laurie E
Process Measurements Division, National Institute of Standards & Technology, Gaithersburg, Maryland 20899, USA.
Anal Chem. 2002 Jun 1;74(11):2556-64. doi: 10.1021/ac025528w.
A new technique is described for the concentration and separation of ionic species in solution within microchannels or capillaries. Concentration is achieved by balancing the electrophoretic velocity of an analyte against the bulk flow of solution in the presence of a temperature gradient. With an appropriate buffer, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity, so that the electrophoretic and bulk velocities sum to zero at a unique point, and the analyte will be focused at that point. The technique is demonstrated for a variety of analytes, including fluorescent dyes, amino acids, DNA, proteins, and particles, and is shown to be capable of greater than 10,000-fold concentration of a dilute analyte.
本文描述了一种用于在微通道或毛细管内对溶液中的离子物种进行浓缩和分离的新技术。在存在温度梯度的情况下，通过平衡分析物的电泳速度与溶液的总体流动来实现浓缩。使用合适的缓冲液，温度梯度可以在电泳速度中产生相应的梯度，使得电泳速度和总体速度在一个独特的点处总和为零，并且分析物将聚焦在该点。该技术已针对多种分析物进行了演示，包括荧光染料、氨基酸、DNA、蛋白质和颗粒，并且显示出能够对稀分析物进行超过10000倍的浓缩。
