Dufrane D, Cornu O, Delloye C, Schneider Y J
University Tissue Bank, Université catholique de Louvain, Orthopaedic Research Laboratory, Brussels, Belgium.
Biomaterials. 2002 Jul;23(14):2979-88. doi: 10.1016/s0142-9612(02)00027-3.
Allogenic human fascia lata used in neurosurgery, as dura mater substitute, can be associated with a risk of viral and bacterial transmission. Chemical and physical procedures, developed to inactivate virus and bacteria, have been applied to fascia lata. The aim of this study consists in the evaluation of the biological properties of this treated graft.
Grafts were treated with solvent detergents, freeze-dried for conservation and gamma irradiated (25,000 Gy) for sterilization. The indirect toxicity evaluation was performed by extraction method, according to the International Standard Organization (ISO). First, the cytotoxic effect of each extracts incubated in the presence of human fibroblasts (WI38) was quantitatively assessed by measuring the cell growth, the viability (succinate dehydrogenase activity, MTT), the membrane integrity (uptake of the neutral red by viable cells, NR) as well as the release of lactate dehydrogenase in the culture medium. Second, confocal laser scanning microscopy (CLSM) was used to assess the direct contact between human primary fibroblasts and graft. CLSM was performed at days 3 and 7 after cells loading.
No acute cytotoxicity was observed for chemically processed allografts. Cells loaded on the graft have demonstrated a good growth and spreading.
Human fascia lata secured against conventional and non-conventional agents is a fully biocompatible alternative to the available dural graft materials.
在神经外科手术中用作硬脑膜替代物的同种异体人阔筋膜可能存在病毒和细菌传播风险。已开发出用于使病毒和细菌失活的化学和物理方法，并应用于阔筋膜。本研究的目的在于评估这种处理过的移植物的生物学特性。
移植物用溶剂洗涤剂处理，冷冻干燥保存，并进行γ射线辐照（25,000戈瑞）灭菌。根据国际标准化组织（ISO）的方法，通过萃取法进行间接毒性评估。首先，通过测量细胞生长、活力（琥珀酸脱氢酶活性，MTT）、膜完整性（活细胞摄取中性红，NR）以及培养基中乳酸脱氢酶的释放，定量评估在人成纤维细胞（WI38）存在下孵育的每种提取物的细胞毒性作用。其次，使用共聚焦激光扫描显微镜（CLSM）评估人原代成纤维细胞与移植物之间的直接接触。在细胞接种后第3天和第7天进行CLSM检测。
化学处理的同种异体移植物未观察到急性细胞毒性。接种在移植物上的细胞生长和铺展良好。
经过常规和非常规制剂处理的人阔筋膜是现有硬脑膜移植物材料的完全生物相容性替代品。
