Freeman Angela B, Duong K Kellye, Shi Tie-Liu, Hughes Carolyn F, Perlin Michael H
Department of Biology, University of Louisville, Louisville, Belknap-LS139, Louisville, KY 40208, USA.
Mol Phylogenet Evol. 2002 May;23(2):158-70. doi: 10.1016/S1055-7903(02)00003-9.
Microbotryum violaceum is a basidiomycete that infects the anthers of its Caryophyllaceae host species. Individual fungal isolates are host limited, though they are not morphologically distinct. This study used variable regions of the highly conserved gamma-tubulin, beta-tubulin, and ribosomal RNA-encoding genes to determine the relationships among M. violaceum fungal isolates from different host species and different geographical locations. Phylogenetic trees from intron nucleotide sequences in two protein-coding genes were compared to trees produced from internal transcribed spacers (ITS) of rDNA. Phylogenetic analyses suggested that there are two clades, one from North America and one from Europe. Isolates from both clades grouped according to host species, although in some analyses isolates from closely related host species were placed together. These results are consistent with the view that M. violaceum has experienced cospeciation with its hosts.
堇菜色微腥黑粉菌是一种担子菌，可感染其石竹科寄主植物的花药。尽管单个真菌分离株在形态上没有明显差异，但它们具有寄主局限性。本研究利用高度保守的γ-微管蛋白、β-微管蛋白和核糖体RNA编码基因的可变区域，来确定来自不同寄主物种和不同地理位置的堇菜色微腥黑粉菌真菌分离株之间的关系。将两个蛋白质编码基因的内含子核苷酸序列构建的系统发育树，与由核糖体DNA的内部转录间隔区（ITS）构建的树进行比较。系统发育分析表明存在两个进化枝，一个来自北美，一个来自欧洲。两个进化枝的分离株均根据寄主物种进行分组，不过在某些分析中，来自亲缘关系较近的寄主物种的分离株被归在一起。这些结果与堇菜色微腥黑粉菌与其寄主经历了协同物种形成的观点一致。
