公猪及精液参数对液态公猪精液储存五天后活力和顶体完整性的影响。

Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days.

作者信息

Kommisrud E, Paulenz H, Sehested E, Grevle I S

机构信息

Department of Reproduction and Forensic Medicine, Norwegian School of Veterinary Science, Oslo, Norway.

出版信息

Acta Vet Scand. 2002;43(1):49-55. doi: 10.1186/1751-0147-43-49.

DOI:10.1186/1751-0147-43-49

PMID:12071116

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1764181/

Abstract

Ninety ejaculates from a total of 76 AI boars were extended in Beltsville Thawing Solution (BTS). Boar identity, breed, weight of the ejaculate and sperm concentration were registered. Motility and acrosome integrity were assessed after storage at 16-18 degrees C for 6, 30, 54, 78, and 102 h. Storage time had a significant influence on both motility (p < 0.01) and acrosome integrity (p < 0.001). The Least Square Means for percentage of motility showed a small decline from 79.8% after 6 h of storage to 78.4% at 102 h. Motility at 78 and 102 h was significantly different from motility at 6 h (p < 0.05). The percentage of sperm cells with normal acrosomes declined throughout the experiment. The Least Square Means for 6, 30, 54, 78, and 102 h of storage were 93.9%, 90.6%, 88.0%, 84.8%, and 78.2%, respectively. The decrease in acrosome integrity from one storage time to the next was highly significant throughout the trial (p < 0.001). There was a significant influence of boar (p < 0.001) and sperm concentration (p < 0.01) on motility, while acrosome integrity was affected only by boar (p < 0.001). Breed of the boars and weight of the ejaculate did not influence the dependent variables.

摘要

从76头人工授精公猪采集的90份精液用贝尔茨维尔解冻液（BTS）进行了稀释。记录了公猪的身份、品种、精液重量和精子浓度。在16 - 18摄氏度下储存6、30、54、78和102小时后，评估了精子活力和顶体完整性。储存时间对精子活力（p < 0.01）和顶体完整性（p < 0.001）均有显著影响。活力百分比的最小二乘均值从储存6小时后的79.8%略有下降至102小时时的78.4%。78小时和102小时时的精子活力与6小时时的活力有显著差异（p < 0.05）。在整个实验过程中，具有正常顶体的精子细胞百分比下降。储存6、30、54、78和102小时的最小二乘均值分别为93.9%、90.6%、88.0%、84.8%和78.2%。在整个试验中，从一个储存时间到下一个储存时间，顶体完整性的下降非常显著（p < 0.001）。公猪（p < 0.001）和精子浓度（p < 0.01）对精子活力有显著影响，而顶体完整性仅受公猪影响（p < 0.001）。公猪品种和精液重量对这些因变量没有影响。